SPARCL1 Polyclonal antibody

SPARCL1 Polyclonal Antibody for WB, IP, IHC, ELISA
Cat No. 13517-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human and More (1)

应用

WB, IP, IHC, ELISA and More (1)

Hevin, MAST 9, PIG33, SC1, SPARC like 1 (hevin), SPARC like protein 1, SPARCL1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inhuman heart tissue
Positive IP detected inRaji cells
Positive IHC detected inhuman tonsillitis tissue, human gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13517-1-AP targets SPARCL1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IP, IHC, ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human
文献引用反应性human, mouse
免疫原 SPARCL1 fusion protein Ag4448 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 SPARC-like 1 (hevin)
别名 Hevin, MAST 9, PIG33, SC1, SPARC like 1 (hevin), SPARC like protein 1, SPARCL1
计算分子量 664 aa, 75 kDa
观测分子量 130-140 kDa
GenBank蛋白编号BC033721
基因名称 SPARCL1
Gene ID (NCBI) 8404
RRIDAB_10598475
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ14515
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SPARC-like protein 1 (SPARCL1), also known as SC1 or Hevin, is an extracellular matrix glycoprotein that belongs to the SPARC family. SPARCL1 is widely expressed in normal and cancer tissues, and has been shown to regulate cellular adhesion and migration. SPARCL1 has been implicated in tumor initiation, formation, and progression of various cancers, including pancreatic, prostate, bladder, ovarian, breast and non-small cell lung cancers.

实验方案

Product Specific Protocols
WB protocol for SPARCL1 antibody 13517-1-APDownload protocol
IHC protocol for SPARCL1 antibody 13517-1-APDownload protocol
IP protocol for SPARCL1 antibody 13517-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

CNS Neurosci Ther

Endothelial genetic deletion of CD147 induces changes in the dual function of the blood-brain barrier and is implicated in Alzheimer's disease.

Authors - Hao Wang

J Neurochem

Defective Synthesis and Release of Astrocytic Thrombospondin-1 Mediates the Neuronal TDP-43 Proteinopathy Resulting in Defects in Neuronal Integrity Associated with Chronic Traumatic Encephalopathy: In Vitro Studies.

Authors - A R Jayakumar
humanIHC

Cancer Biomark

Integrated analysis reveals down-regulation of SPARCL1 is correlated with cervical cancer development and progression

Authors - Dong-Ming Wu
humanWB

Oral Dis

FERMT2 upregulation in CAFs enhances EMT of OSCC and M2 macrophage polarization

Authors - Xiangrui Ma
mouseIF

PLoS One

Secreted protein acidic and rich in cysteine (SPARC) knockout mice have greater outflow facility.

Authors - Ling Yu
mouseWB

J Chem Neuroanat

Olfactory ensheathing cells facilitate neurite sprouting and outgrowth by secreting high levels of hevin.

Authors - Lite Ge
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