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SPANXE Polyclonal antibody

SPANXE Polyclonal Antibody for IF/ICC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC, WB, ELISA

Conjugate

Unconjugated

Cat No : 15870-1-AP

Print datasheet

Synonyms

SPANX A2, SPANX C, SPANX D, SPANX E, SPANX family member E, SPANX family, member E, SPANXA2, SPANXD, SPANXE

验证数据展示

  • WB analysis of A375 using 15870-1-AP

    WB analysis of A375 using 15870-1-AP

    A375 cells were subjected to SDS PAGE followed by western blot with 15870-1-AP (SPANXE antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IF Staining of A375 using 15870-1-AP

    IF Staining of A375 using 15870-1-AP

    Immunofluorescent analysis of A375 cells using 15870-1-AP (SPANXE antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inA375 cells, mouse skin tissue
Positive IF detected inA375 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15870-1-AP targets SPANXE in WB, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IF/ICC, WB, ELISA
Tested Reactivity human, mouse
Immunogen SPANXE fusion protein Ag8572 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name SPANX family, member E
Synonyms SPANX A2, SPANX C, SPANX D, SPANX E, SPANX family member E, SPANX family, member E, SPANXA2, SPANXD, SPANXE
Calculated Molecular Weight 97 aa, 11 kDa
Observed Molecular Weight 15-20 kDa
GenBank Accession NumberBC005382
Gene Symbol SPANXE
Gene ID (NCBI) 171489
RRIDAB_10951107
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8TAD1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SPANXE (Sperm protein associated with the nucleus on the X chromosome E), also known as SPANX A2 or SPANX C, is a member of the SPANX family. The SPANX genes encode differentially expressed testis-specific proteins that localize to various subcellular compartments. This particular gene encodes a sperm protein that contains a consensus nuclear localization signal but, although a role in spermatogenesis is suggested, the specific function of this family member has not yet been determined.

实验方案

Product Specific Protocols
WB protocol for SPANXE antibody 15870-1-APDownload protocol
IF protocol for SPANXE antibody 15870-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols