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验证数据展示

  • WB analysis using 14726-1-AP (same clone as 14726-1-PBS)

    WB analysis using 14726-1-AP (same clone as 14726-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 14726-1-AP (SPAG5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14726-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 14726-1-AP (same clone as 14726-1-PBS)

    IP experiment of HeLa using 14726-1-AP (same clone as 14726-1-PBS)

    IP result of anti-SPAG5 (IP:14726-1-AP, 5ug; Detection:14726-1-AP 1:1000) with HeLa cells lysate 3000ug. This data was developed using the same antibody clone with 14726-1-PBS in a different storage buffer formulation.

  • IHC staining of human testis using 14726-1-AP (same clone as 14726-1-PBS)

    IHC staining of human testis using 14726-1-AP (same clone as 14726-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis using 14726-1-AP (SPAG5 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 14726-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SPAG5 antibody included
  • IHC staining of human testis using 14726-1-AP (same clone as 14726-1-PBS)

    IHC staining of human testis using 14726-1-AP (same clone as 14726-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis using 14726-1-AP (SPAG5 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 14726-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SPAG5 antibody included
  • IF Staining of HeLa using 14726-1-AP (same clone as 14726-1-PBS)

    IF Staining of HeLa using 14726-1-AP (same clone as 14726-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using SPAG5 antibody (14726-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14726-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 14726-1-AP (same clone as 14726-1-PBS)

    IF Staining of HeLa using 14726-1-AP (same clone as 14726-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using SPAG5 antibody (14726-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 14726-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

SPAG5 Polyclonal antibody, PBS Only

SPAG5 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 14726-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

sperm associated antigen 5, Mitotic spindle-associated protein p126, MAP126, hMAP126, Deepest

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy SPAG5 Ready-To-Use IHC Kit. SPAG5 primary antibody included.

产品信息

14726-1-PBS targets SPAG5 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag6485

Product name: Recombinant human SPAG5 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 844-1193 aa of BC000322

Sequence: TVENLTAKLASTIADNQEQDLEKTRQYSQKLGLLTEQLQSLTLFLQTKLKEKTEQETLLLSTACPPTQEHPLPNDRTFLGSILTAVADEEPESTPVPLLGSDKSAFTRVASMVSLQPAETPGMEESLAEMSIMTTELQSLCSLLQESKEEAIRTLQRKICELQARLQAQEEQHQEVQKAKEADIEKLNQALCLRYKNEKELQEVIQQQNEKILEQIDKSGELISLREEVTHLTRSLRRAETETKVLQEALAGQLDSNCQPMATNWIQEKVWLSQEVDKLRVMFLEMKNEKEKLMIKFQSHRNILEENLRRSDKELEKLDDIVQHIYKTLLSIPEVVRGCKELQGLLEFLS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 sperm associated antigen 5
别名 sperm associated antigen 5, Mitotic spindle-associated protein p126, MAP126, hMAP126, Deepest
计算分子量 134 kDa
观测分子量 135 kDa, 150 kDa
GenBank蛋白编号BC000322
基因名称 SPAG5
Gene ID (NCBI) 10615
RRIDAB_2194787
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96R06
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SPAG5, also named as MAP126, Astrin and Deepest, is necessary for normal spindle formation during mitosis. It is highly expressed in testis. SPAG5 has different localization in somatic versus male germ cells suggesting the possibility of different function. This antibody is specific to SPAG5.

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