验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells, HeLa cells, K-562 cells, PC-3 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human testis tissue, mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
14726-1-AP targets SPAG5 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse |
| 免疫原 | SPAG5 fusion protein Ag6485 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | sperm associated antigen 5 |
| 别名 | sperm associated antigen 5, Mitotic spindle-associated protein p126, MAP126, hMAP126, Deepest |
| 计算分子量 | 134 kDa |
| 观测分子量 | 135 kDa, 150 kDa |
| GenBank蛋白编号 | BC000322 |
| 基因名称 | SPAG5 |
| Gene ID (NCBI) | 10615 |
| RRID | AB_2194787 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q96R06 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SPAG5, also named as MAP126, Astrin and Deepest, is necessary for normal spindle formation during mitosis. It is highly expressed in testis. SPAG5 has different localization in somatic versus male germ cells suggesting the possibility of different function. This antibody is specific to SPAG5.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SPAG5 antibody 14726-1-AP | Download protocol |
| IHC protocol for SPAG5 antibody 14726-1-AP | Download protocol |
| IF protocol for SPAG5 antibody 14726-1-AP | Download protocol |
| IP protocol for SPAG5 antibody 14726-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun CDC20B is required for deuterosome-mediated centriole production in multiciliated cells. | ||
Elife Centriolar satellites assemble centrosomal microcephaly proteins to recruit CDK2 and promote centriole duplication.
| ||
Elife Kinetochores attached to microtubule-ends are stabilised by Astrin bound PP1 to ensure proper chromosome segregation. | ||
Elife CLUH controls astrin-1 expression to couple mitochondrial metabolism to cell cycle progression. | ||
Elife DIAPH3 deficiency links microtubules to mitotic errors, defective neurogenesis, and brain dysfunction. | ||
J Nanobiotechnology Fe-doped chrysotile nanotubes containing siRNAs to silence SPAG5 to treat bladder cancer. |
