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验证数据展示

  • WB analysis using 14726-1-AP

    WB analysis using 14726-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14726-1-AP (SPAG5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IP experiment of HeLa using 14726-1-AP

    IP experiment of HeLa using 14726-1-AP

    IP result of anti-SPAG5 (IP:14726-1-AP, 5ug; Detection:14726-1-AP 1:1000) with HeLa cells lysate 3000ug.

  • IHC staining of human testis using 14726-1-AP

    IHC staining of human testis using 14726-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 14726-1-AP (SPAG5 antibody) at dilution of 1:100 (under 10x lens).

    Ready-to-use IHC kits available with SPAG5 antibody included
  • IHC staining of human testis using 14726-1-AP

    IHC staining of human testis using 14726-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 14726-1-AP (SPAG5 antibody) at dilution of 1:100 (under 40x lens).

    Ready-to-use IHC kits available with SPAG5 antibody included
  • IF Staining of HeLa using 14726-1-AP

    IF Staining of HeLa using 14726-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using SPAG5 antibody (14726-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IHC staining of human placenta using 14726-1-AP

    IHC staining of human placenta using 14726-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 14726-1-AP (SPAG5 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SPAG5 antibody included
  • IHC staining of human stomach cancer using 14726-1-AP

    IHC staining of human stomach cancer using 14726-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 14726-1-AP (SPAG5 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SPAG5 antibody included
  • IHC staining of mouse kidney using 14726-1-AP

    IHC staining of mouse kidney using 14726-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 14726-1-AP (SPAG5 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SPAG5 antibody included
  • IHC staining of mouse liver using 14726-1-AP

    IHC staining of mouse liver using 14726-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 14726-1-AP (SPAG5 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SPAG5 antibody included
  • IHC staining of mouse thymus using 14726-1-AP

    IHC staining of mouse thymus using 14726-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse thymus tissue slide using 14726-1-AP (SPAG5 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SPAG5 antibody included
  • IF Staining of HeLa using 14726-1-AP

    IF Staining of HeLa using 14726-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using SPAG5 antibody (14726-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Featured Product
  • KD/KO Validated

SPAG5 Polyclonal antibody

SPAG5 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 14726-1-AP

产品说明书

引用文献(25)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, IP, ELISA and More (1)

别名

Astrin, Deepest, hMAP126, MAP126, Mitotic spindle-associated protein p126

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inA549 cells, HeLa cells, K-562 cells, PC-3 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman testis tissue, mouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy SPAG5 Ready-To-Use IHC Kit. SPAG5 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14726-1-AP targets SPAG5 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用WB, IHC, IF, IP
经测试反应性 human, mouse
文献引用反应性human, mouse
免疫原

CatNo: Ag6485

Product name: Recombinant human SPAG5 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 844-1193 aa of BC000322

Sequence: TVENLTAKLASTIADNQEQDLEKTRQYSQKLGLLTEQLQSLTLFLQTKLKEKTEQETLLLSTACPPTQEHPLPNDRTFLGSILTAVADEEPESTPVPLLGSDKSAFTRVASMVSLQPAETPGMEESLAEMSIMTTELQSLCSLLQESKEEAIRTLQRKICELQARLQAQEEQHQEVQKAKEADIEKLNQALCLRYKNEKELQEVIQQQNEKILEQIDKSGELISLREEVTHLTRSLRRAETETKVLQEALAGQLDSNCQPMATNWIQEKVWLSQEVDKLRVMFLEMKNEKEKLMIKFQSHRNILEENLRRSDKELEKLDDIVQHIYKTLLSIPEVVRGCKELQGLLEFLS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 sperm associated antigen 5
别名 Astrin, Deepest, hMAP126, MAP126, Mitotic spindle-associated protein p126
计算分子量 134 kDa
观测分子量 135 kDa, 150 kDa
GenBank蛋白编号BC000322
基因名称 SPAG5
Gene ID (NCBI) 10615
RRIDAB_2194787
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96R06
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SPAG5, also named as MAP126, Astrin and Deepest, is necessary for normal spindle formation during mitosis. It is highly expressed in testis. SPAG5 has different localization in somatic versus male germ cells suggesting the possibility of different function. This antibody is specific to SPAG5.

实验方案

Product Specific Protocols
IF protocol for SPAG5 antibody 14726-1-APDownload protocol
IHC protocol for SPAG5 antibody 14726-1-APDownload protocol
IP protocol for SPAG5 antibody 14726-1-APDownload protocol
WB protocol for SPAG5 antibody 14726-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouse,humanWB,IF

Nat Commun

CDC20B is required for deuterosome-mediated centriole production in multiciliated cells.

Authors - Diego R Revinski
View Article
humanWB,IHC

J Nanobiotechnology

Fe-doped chrysotile nanotubes containing siRNAs to silence SPAG5 to treat bladder cancer.

Authors - Jianye Liu
View Article
mouseWB,IF

MedComm (2020)

Host Translational Control by Stress Granules Promotes Mycobacterium tuberculosis Pathogenesis.

Authors - Jaewhan Kim
View Article
humanWB

Cell Death Discov

The splicing factor SF3B4 drives proliferation and invasion in cervical cancer by regulating SPAG5

Authors - Yingwei Li
  • KD Validated
View Article
humanWB,IHC

Apoptosis

SPAG5 promotes ferroptosis and enhances chemotherapy efficacy in locally advanced colorectal cancer via upregulating intracellular ROS levels.

Authors - Ruiqi Gu
  • KD Validated
View Article
WB

Cell Rep

KIF18A promotes chromosome congression in cooperation with CENP-E downstream of CENP-C.

Authors - Jiahang Miao
View Article
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