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验证数据展示

  • IF Staining of HepG2 using 19721-1-AP (same clone as 19721-1-PBS)

    IF Staining of HepG2 using 19721-1-AP (same clone as 19721-1-PBS)

    Immunofluorescent analysis of HepG2 cells using 19721-1-AP (SPAG4-Specific antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 19721-1-PBS in a different storage buffer formulation.

  • WB analysis of human testis using 19721-1-AP (same clone as 19721-1-PBS)

    WB analysis of human testis using 19721-1-AP (same clone as 19721-1-PBS)

    human testis tissue were subjected to SDS PAGE followed by western blot with 19721-1-AP (SPAG4-Specific Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19721-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse testis using 19721-1-AP (same clone as 19721-1-PBS)

    IF Staining of mouse testis using 19721-1-AP (same clone as 19721-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using SPAG4-Specific antibody (19721-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 19721-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse testis using 19721-1-AP (same clone as 19721-1-PBS)

    IHC staining of mouse testis using 19721-1-AP (same clone as 19721-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 19721-1-AP (SPAG4-Specific antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19721-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse testis using 19721-1-AP (same clone as 19721-1-PBS)

    IHC staining of mouse testis using 19721-1-AP (same clone as 19721-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 19721-1-AP (SPAG4-Specific antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19721-1-PBS in a different storage buffer formulation.

SPAG4-Specific Polyclonal antibody, PBS Only

SPAG4-Specific Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA
Cat No. 19721-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, IF-P, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

19721-1-PBS targets SPAG4-Specific in WB, IHC, IF/ICC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, IF-P, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 sperm associated antigen 4
别名
计算分子量 48 kDa
观测分子量 55 kDa
GenBank蛋白编号NM_003116
基因名称 SPAG4
Gene ID (NCBI) 6676
RRIDAB_2878600
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NPE6
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SPAG4 may assist the organization and assembly of outer dense fibers (ODFs), a specific structure of the sperm tail. This antibody is specific to SPAG4. We observed that the molecular weight of SPAG4-Specific was 55 kDa.

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