验证数据展示
经过测试的应用
| Positive WB detected in | human testis tissue, HepG2 cells |
| Positive IHC detected in | mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse testis tissue |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 3 publications below |
产品信息
19721-1-AP targets SPAG4-Specific in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, IF/ICC, IF-P, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse |
| 免疫原 | Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | sperm associated antigen 4 |
| 别名 | SPAG4, Outer dense fiber-associated protein SPAG4, SUN4, SUN domain-containing protein 4, Sperm-associated antigen 4 protein |
| 计算分子量 | 48 kDa |
| 观测分子量 | 55 kDa |
| GenBank蛋白编号 | NM_003116 |
| 基因名称 | SPAG4 |
| Gene ID (NCBI) | 6676 |
| RRID | AB_2878600 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9NPE6 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SPAG4 may assist the organization and assembly of outer dense fibers (ODFs), a specific structure of the sperm tail. This antibody is specific to SPAG4. We observed that the molecular weight of SPAG4-Specific was 55 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SPAG4-Specific antibody 19721-1-AP | Download protocol |
| IHC protocol for SPAG4-Specific antibody 19721-1-AP | Download protocol |
| IF protocol for SPAG4-Specific antibody 19721-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Biol Chem The testis-specific LINC component SUN3 is essential for sperm head shaping during mouse spermiogenesis. | ||
Cancer Cell Int Novel risk model based on angiogenesis-related lncRNAs for prognosis prediction of hepatocellular carcinoma | ||
J Biochem Mol Toxicol SPAG4 enhances mitochondrial respiration and aerobic glycolysis in colorectal cancer cells by activating the PI3K/Akt signaling pathway |
