×
Use Able AI chat for product recommendations

验证数据展示

  • WB analysis of rat testis using 16883-1-AP (same clone as 16883-1-PBS)

    WB analysis of rat testis using 16883-1-AP (same clone as 16883-1-PBS)

    rat testis tissue were subjected to SDS PAGE followed by western blot with 16883-1-AP (SPAG16 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16883-1-PBS in a different storage buffer formulation.

  • IHC staining of human brain using 16883-1-AP (same clone as 16883-1-PBS)

    IHC staining of human brain using 16883-1-AP (same clone as 16883-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain using 16883-1-AP (SPAG16 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 16883-1-PBS in a different storage buffer formulation.

  • IHC staining of human brain using 16883-1-AP (same clone as 16883-1-PBS)

    IHC staining of human brain using 16883-1-AP (same clone as 16883-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain using 16883-1-AP (SPAG16 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 16883-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 16883-1-AP (same clone as 16883-1-PBS)

    IF Staining of HepG2 using 16883-1-AP (same clone as 16883-1-PBS)

    Immunofluorescent analysis of HepG2 cells, using SPAG16 antibody 16883-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye). This data was developed using the same antibody clone with 16883-1-PBS in a different storage buffer formulation.

  • IP experiment of mouse testis using 16883-1-AP (same clone as 16883-1-PBS)

    IP experiment of mouse testis using 16883-1-AP (same clone as 16883-1-PBS)

    IP result of anti-SPAG16 (IP:16883-1-AP, 4ug; Detection:16883-1-AP 1:1000) with mouse testis tissue lysate 4000ug. This data was developed using the same antibody clone with 16883-1-PBS in a different storage buffer formulation.

SPAG16 Polyclonal antibody, PBS Only

SPAG16 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 16883-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

点击咨询售前客服

点击咨询售后客服

产品信息

16883-1-PBS targets SPAG16 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag10348

Product name: Recombinant human SPAG16 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-181 aa of BC009614

Sequence: MAAQRGMPSSAVRVLEEALGMGLTAAGDARDTADAVAAEGAYYLEQVTITEASEDDYEYEEIPDDNFSIPEGEEDLAKAIQMAQEQATDTEILERKTVLPSKHAVPEVIEDFLCNFLIKMGMTRTLDCFQSEWYELIQKGVTELRTVGNVPDVYTQIMLLENENKNLKKDLKHYKQAAEYV

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 sperm associated antigen 16
别名
计算分子量 631 aa, 71 kDa
观测分子量 71 kDa
GenBank蛋白编号BC009614
基因名称 SPAG16
Gene ID (NCBI) 79582
RRIDAB_2194658
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8N0X2
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SPAG16 (also known as PF20) is an orthologue of Chlamydomonas PF20, a component of the alga axoneme central apparatus that is required for flagellar motility. SPAG16 is highly expressed in the testis, low abundant or absent from other tissues. The SPAG16 gene encodes two major proteins, a 71-kDa SPAG16L and a smaller 35-kDa SPAG16S nuclear protein that represents the C-terminus of SPAG16L. SPAG16L was detected abundantly in the cytoplasm, while SPAG16S was detected in both cytoplasm and nucleus. This antibody was raised against the N-terminal region of SPAG16 and mainly recognizes SPAG16L.

||
New chat

Able™

正在加载，请稍候...