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SPAG16 Polyclonal antibody

SPAG16 Polyclonal Antibody for IF/ICC, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, IHC, IP, WB,ELISA

Conjugate

Unconjugated

Cat No : 16883-1-AP

Print datasheet

Synonyms

PF20, Pf20 protein homolog, SPAG16, sperm associated antigen 16, WDR29

验证数据展示

  • WB analysis of rat testis using 16883-1-AP

    WB analysis of rat testis using 16883-1-AP

    rat testis tissue were subjected to SDS PAGE followed by western blot with 16883-1-AP (SPAG16 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human brain using 16883-1-AP

    IHC staining of human brain using 16883-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 16883-1-AP (SPAG16 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human brain using 16883-1-AP

    IHC staining of human brain using 16883-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 16883-1-AP (SPAG16 antibody) at dilution of 1:100 (under 40x lens).

  • IF Staining of HepG2 using 16883-1-AP

    IF Staining of HepG2 using 16883-1-AP

    Immunofluorescent analysis of HepG2 cells, using SPAG16 antibody 16883-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

  • IP experiment of mouse testis using 16883-1-AP

    IP experiment of mouse testis using 16883-1-AP

    IP result of anti-SPAG16 (IP:16883-1-AP, 4ug; Detection:16883-1-AP 1:1000) with mouse testis tissue lysate 4000ug.

经过测试的应用

Positive WB detected inrat testis tissue, mouse testis tissue
Positive IP detected inmouse testis tissue
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

16883-1-AP targets SPAG16 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, IHC, IP, WB,ELISA
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen SPAG16 fusion protein Ag10348 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name sperm associated antigen 16
Synonyms PF20, Pf20 protein homolog, SPAG16, sperm associated antigen 16, WDR29
Calculated Molecular Weight 631 aa, 71 kDa
Observed Molecular Weight 71 kDa
GenBank Accession NumberBC009614
Gene Symbol SPAG16
Gene ID (NCBI) 79582
RRIDAB_2194658
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8N0X2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SPAG16 (also known as PF20) is an orthologue of Chlamydomonas PF20, a component of the alga axoneme central apparatus that is required for flagellar motility. SPAG16 is highly expressed in the testis, low abundant or absent from other tissues. The SPAG16 gene encodes two major proteins, a 71-kDa SPAG16L and a smaller 35-kDa SPAG16S nuclear protein that represents the C-terminus of SPAG16L. SPAG16L was detected abundantly in the cytoplasm, while SPAG16S was detected in both cytoplasm and nucleus. This antibody was raised against the N-terminal region of SPAG16 and mainly recognizes SPAG16L.

实验方案

Product Specific Protocols
WB protocol for SPAG16 antibody 16883-1-APDownload protocol
IHC protocol for SPAG16 antibody 16883-1-APDownload protocol
IF protocol for SPAG16 antibody 16883-1-APDownload protocol
IP protocol for SPAG16 antibody 16883-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Ecotoxicol Environ Saf

Microcystin leucine arginine induces human sperm damage: Involvement of the Ca2+/CaMKKβ/AMPK pathway

Authors - Xing Guo
View Article