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验证数据展示

  • WB analysis using 13367-1-AP (same clone as 13367-1-PBS)

    WB analysis using 13367-1-AP (same clone as 13367-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 13367-1-AP (SPA17 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13367-1-PBS in a different storage buffer formulation.

  • IP experiment of mouse testis using 13367-1-AP (same clone as 13367-1-PBS)

    IP experiment of mouse testis using 13367-1-AP (same clone as 13367-1-PBS)

    IP result of anti-SPA17 (IP:13367-1-AP, 4ug; Detection:13367-1-AP 1:5000) with mouse testis tissue lysate 1520 ug. This data was developed using the same antibody clone with 13367-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse testis using 13367-1-AP (same clone as 13367-1-PBS)

    IHC staining of mouse testis using 13367-1-AP (same clone as 13367-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13367-1-AP (SPA17 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13367-1-PBS in a different storage buffer formulation.

SPA17 Polyclonal antibody, PBS Only

SPA17 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 13367-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IP, Indirect ELISA

别名

CT22, SP17, SP17 1, Sp17-1, SPA 17

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

13367-1-PBS targets SPA17 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag4186

Product name: Recombinant human SPA17 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-151 aa of BC032457

Sequence: MSIPFSNTHYRIPQGFGNLLEGLTREILREQPDNIPAFAAAYFESLLEKREKTNFDPAEWGSKVEDRFYNNHAFEEQEPPEKSDPKQEESQISGKEEETSVTILDSSEEDKEKEEVAAVKIQAAFRGHIAREEAKKMKTNSLQNEEKEENK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 sperm autoantigenic protein 17
别名 CT22, SP17, SP17 1, Sp17-1, SPA 17
计算分子量 151 aa, 17 kDa
观测分子量 25 kDa
GenBank蛋白编号BC032457
基因名称 SPA17
Gene ID (NCBI) 53340
RRIDAB_2194631
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ15506
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Sperm surface protein Sp17(SPA17)is a 151 amino acids protein (17kDa), which is a sperm surface peripheral membrane protein. It is a highly conserved mammalian protein expressed in developing spermatozoa and mature spermatids, and reported to play a role in the interaction of sperm with the zona pellucida. SP17 exists as a homodimer and localizes to the head and tail of spermatozoa. SP17 has been identified as a cancer/testis antigen and is expressed in ovarian cancer and multiple myeloma. This suggests that SP17 could be suitable as a target in tumor immunotherapy.

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