SPA17 Polyclonal antibody
SPA17 Polyclonal Antibody for IHC, IP, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
IHC, IP, WB,ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | mouse testis tissue |
| Positive IP detected in | mouse testis tissue |
| Positive IHC detected in | human testis tissue, human ovary tumor tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2400 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
13367-1-AP targets SPA17 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | IHC, IP, WB,ELISA |
| Cited Applications | WB, IF, IHC |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Immunogen | SPA17 fusion protein Ag4186 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | sperm autoantigenic protein 17 |
| Synonyms | Cancer/testis antigen 22, CT22, SP17, SP17 1, SPA17, sperm autoantigenic protein 17, Sperm protein 17, Sperm surface protein Sp17 |
| Calculated Molecular Weight | 151 aa, 17 kDa |
| Observed Molecular Weight | 25 kDa |
| GenBank Accession Number | BC032457 |
| Gene Symbol | SPA17 |
| Gene ID (NCBI) | 53340 |
| RRID | AB_2194631 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q15506 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Sperm surface protein Sp17(SPA17)is a 151 amino acids protein (17kDa), which is a sperm surface peripheral membrane protein. It is a highly conserved mammalian protein expressed in developing spermatozoa and mature spermatids, and reported to play a role in the interaction of sperm with the zona pellucida. SP17 exists as a homodimer and localizes to the head and tail of spermatozoa. SP17 has been identified as a cancer/testis antigen and is expressed in ovarian cancer and multiple myeloma. This suggests that SP17 could be suitable as a target in tumor immunotherapy.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SPA17 antibody 13367-1-AP | Download protocol |
| IHC protocol for SPA17 antibody 13367-1-AP | Download protocol |
| IP protocol for SPA17 antibody 13367-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Br J Cancer Tumour antigen expression in hepatocellular carcinoma in a low-endemic western area. | ||
Front Immunol Sperm Autoantigenic Protein 17 Predicts the Prognosis and the Immunotherapy Response of Cancers: A Pan-Cancer Analysis. | ||
Sci Rep Testis-specific peroxiredoxin 4 variant is not absolutely required for spermatogenesis and fertility in mice. | ||
BMC Cancer Validity and prognostic significance of sperm protein 17 as a tumor biomarker for epithelial ovarian cancer: a retrospective study. | ||
Asian J Androl Decreased AKAP4/PKA signaling pathway in high DFI sperm affects sperm capacitation |
