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验证数据展示

  • WB analysis of fetal human brain using 21234-1-AP (same clone as 21234-1-PBS)

    WB analysis of fetal human brain using 21234-1-AP (same clone as 21234-1-PBS)

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 21234-1-AP (KLF14 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21234-1-PBS in a different storage buffer formulation.

  • IHC staining of human skin using 21234-1-AP (same clone as 21234-1-PBS)

    IHC staining of human skin using 21234-1-AP (same clone as 21234-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skin using 21234-1-AP (KLF14 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 21234-1-PBS in a different storage buffer formulation.

  • IHC staining of human skin using 21234-1-AP (same clone as 21234-1-PBS)

    IHC staining of human skin using 21234-1-AP (same clone as 21234-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skin using 21234-1-AP (KLF14 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 21234-1-PBS in a different storage buffer formulation.

  • IF Staining of Caco-2 using 21234-1-AP (same clone as 21234-1-PBS)

    IF Staining of Caco-2 using 21234-1-AP (same clone as 21234-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Caco-2 cells using SP6 antibody (21234-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 21234-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

SP6 Polyclonal antibody, PBS Only

SP6 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 21234-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

AI1K, EPFN, EPIPROFIN, KLF14, Krueppel-like factor 14

缓冲液配方: 
偶联物:  Unconjugated
规格价格库存

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产品信息

21234-1-PBS targets SP6 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag15707

Product name: Recombinant human SP6 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-235 aa of BC103951

Sequence: MLTAVCGSLGSQHTEAPHASPPRLDLQPLQTYQGHTSPEAGDYPSPLQPGELQSLPLGPEVDFSQGYELPGASSRVTCEDLESDSPLAPGPFSKLLQPDMSHHYESWFRPTHPGAEDGSWWDLHPGTSWMDLPHTQGALTSPGHPGALQAGLGGYVGDHQLCAPPPHPHAHHLLPAAGGQHLLGPPDGAKALEVAAPESQGLDSSLDGAARPKGSRRSVPRSSGQTVCRCPNCLE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 Sp6 transcription factor
别名 AI1K, EPFN, EPIPROFIN, KLF14, Krueppel-like factor 14
计算分子量 376 aa, 40 kDa
GenBank蛋白编号BC103951
基因名称 SP6
Gene ID (NCBI) 80320
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ3SY56
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SP6 (chromosome 17) is a 376 amino acid protein, which localizes in nucleus. SP6 is a transcription factor of the SP/KLF family and an indispensable regulator of the morphological dynamics of ameloblast differentiation during tooth development. The levels of sp6 protein expressed in various tissues in the embryo. This antibody detects the SP6 protein. It can't detect the KLF14/BTEB5.

This antibody was previously listed as a KLF14 antibody, because the SP6 gene on chromosome 17q21.32 has been referred to as KLF14 in the literature. SP6 should not be confused with the KLF14 gene on chromosome 7q32.2.

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