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验证数据展示

  • WB analysis of MCF-7 using 83660-1-RR (same clone as 83660-1-PBS)

    WB analysis of MCF-7 using 83660-1-RR (same clone as 83660-1-PBS)

    WB result of SOX4 antibody (83660-1-RR; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SOX4 transfected MCF-7 cells. This data was developed using the same antibody clone with 83660-1-PBS in a different storage buffer formulation.

  • FC experiment of SH-SY5Y using 83660-1-RR (same clone as 83660-1-PBS)

    FC experiment of SH-SY5Y using 83660-1-RR (same clone as 83660-1-PBS)

    1x10^6 SH-SY5Y cells were intracellularly stained with 0.25 ug SOX4 Recombinant antibody (83660-1-RR, Clone:240625E3) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 83660-1-PBS in a different storage buffer formulation.

  • WB analysis using 83660-1-RR (same clone as 83660-1-PBS)

    WB analysis using 83660-1-RR (same clone as 83660-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83660-1-RR (SOX4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83660-1-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP00619-1

    Sandwich ELISA standard curve of MP00619-1

    Sandwich ELISA standard curve of MP00619-1, Human SOX4 Recombinant Matched Antibody Pair - PBS only. 83660-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag26583. 83660-3-PBS was HRP conjugated as the detection antibody. Range: 31.3-2000 pg/mL.

  • Sample test of MP00619-1

    Sample test of MP00619-1

    The mean SOX4 concentration was determined to be 16.1 ng/mL in HepG2 cell extract based on a 1.2 mg/mL extract load.

  • Cytometric bead array standard curve of MP00619-1

    Cytometric bead array standard curve of MP00619-1

    Cytometric bead array standard curve of MP00619-1, SOX4 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83660-1-PBS. Detection antibody: 83660-3-PBS. Standard: Ag26583. Range: 0.625-80 ng/mL.

  • Affinity and Kinetic Characterization of 83660-1-RR

    Affinity and Kinetic Characterization of 83660-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83660-1-RR against Human SOX4 were performed. The affinity constant is below 1 pM.

  • Featured Product
  • KD/KO验证

SOX4 Recombinant antibody, PBS Only (Capture)

SOX4 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 83660-1-PBS

产品说明书

CloneNo. 240625E3

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

别名

240625E3, CSS10, EVI16, Transcription factor SOX-4

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

83660-1-PBS targets SOX4 as part of a matched antibody pair:

MP00619-1: 83660-1-PBS capture and 83660-3-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

MP00619-2: 83660-1-PBS capture and 83660-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Application Description
经测试反应性 human
免疫原 SOX4 fusion protein Ag26583 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 SRY (sex determining region Y)-box 4
别名 240625E3, CSS10, EVI16, Transcription factor SOX-4
计算分子量 474 aa, 47 kDa
观测分子量47 kDa
GenBank蛋白编号BC072668
基因名称 SOX4
Gene ID (NCBI) 6659
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ06945
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SOX4 is a member of the SOX (SRY-related HMG-box) family of transcription factors that play key roles in the regulation of stemness, differentiation, progenitor cell development, and multiple developmental pathways. A single-exon gene encodes the SOX4 protein and contains a highly conserved high-mobility group (HMG) DNA binding domain related to the TCF/LEF family of transcription factors that play important roles in the Wnt pathway. SOX4 is expressed in stem cells, and modulates stem cell activation and likely also plays a role in stem cell maintenance, possibly through activation of expression of SOX2 (PMID: 23764001, 31445218). The calculated molecular weight of SOX4 is 47 kDa.