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验证数据展示

  • IF Staining of U2OS using CL488-83189-3

    IF Staining of U2OS using CL488-83189-3

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using CoraLite® Plus 488 SORBS2 antibody (CL488-83189-3, Clone: 240023A8 ) at dilution of 1:200.

  • FC experiment of U2OS using CL488-83189-3

    FC experiment of U2OS using CL488-83189-3

    1x10^6 U2OS cells were intracellularly stained with 0.4 ug CoraLite® Plus 488-conjugated SORBS2 Recombinant antibody (CL488-83189-3, Clone:240023A8)(red), or 0.4 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

CoraLite® Plus 488-conjugated SORBS2 Recombinant antibody

SORBS2 Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra)
Cat No. CL488-83189-3

产品说明书

CloneNo. 240023A8

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, FC (Intra)

别名

Sorbin and SH3 domain-containing protein 2, ArgBP2, Arg-binding protein 2, Arg/Abl-interacting protein 2, 240023A8

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inU2OS cells
Positive FC (Intra) detected inU2OS cells
Planning an IHC experiment? We recommend our IHCeasy SORBS2 Ready-To-Use IHC Kit. SORBS2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 83189-3-PBS

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-83189-3 targets SORBS2 in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human
免疫原 SORBS2 fusion protein Ag20041 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 sorbin and SH3 domain containing 2
别名 Sorbin and SH3 domain-containing protein 2, ArgBP2, Arg-binding protein 2, Arg/Abl-interacting protein 2, 240023A8
计算分子量 1100 aa, 124 kDa
观测分子量 70-75 kDa
GenBank蛋白编号BC011883
基因名称 SORBS2
Gene ID (NCBI) 8470
RRIDAB_3673218
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO94875
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SORBS2, also known as ARGBP2, has three C-terminal SH3 domains and an N-terminal sorbin homology (SoHo) domain that interacts with lipid raft proteins. SORBS2 is widely expressed in human tissues and extremely abundant in the heart. In epithelial cells SORBS2 is located in stress fibers and the nucleus; In cardiac muscle cells. SORBS2 is located in the Z-disks of sarcomeres. The subcellular localization suggests that SORBS2 functions as an adapter protein to assemble signaling complexes in stress fibers, and may influence the contractile or elastic properties of cardiac sarcomeres(PMID: 9211900). Alternate splicing results in multiple transcript variants( PMID: 28961272).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 SORBS2 antibody CL488-83189-3Download protocol
FC protocol for CL Plus 488 SORBS2 antibody CL488-83189-3Download protocol
Standard Protocols
Click here to view our Standard Protocols