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验证数据展示

  • IHC staining of human testis using 17753-1-AP

    IHC staining of human testis using 17753-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 17753-1-AP (SOHLH1 Antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human testis using 17753-1-AP

    IHC staining of human testis using 17753-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 17753-1-AP (SOHLH1 Antibody) at dilution of 1:100 (under 40x lens).

SOHLH1 Polyclonal antibody

SOHLH1 Polyclonal Antibody for IHC, ELISA
Cat No. 17753-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IHC, ELISA and More (2)

别名

C9orf157, NOHLH, SOHLH1, TEB2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17753-1-AP targets SOHLH1 in WB, IF, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 IHC, ELISA Application Description
文献引用应用WB, IF
经测试反应性 human
文献引用反应性human
免疫原

CatNo: Ag12107

Product name: Recombinant human SOHLH1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-328 aa of BC031861

Sequence: MASRCSEPYPEVSRIPTVRGCNGSLSGALSCCEDSAQGSGPPKAPTVAEGPSSCLRRNVISERERRKRMSLSCERLRALLPQFDGRREDMASVLEMSVQFLRLASALGPSQEQHAILASSKEMWHSLQEDVLQLTLSSQIQAGVPDPGTGASSGTRTPDVKAFLESPWSLDPASASPEPVPHILASSRQWDPASCTSLGTDKCEALLGLCQVRGGLPPFSEPSSLVPWPPGRSLPKAVRPPLSWPPFSQQQTLPVMSGEALGWLGQAGSLAMGAAPLGEPAKEDPMLAQEAGSALGSDVDDGTSFLLTAGPSSWPGEWGPGFRAGPPA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 spermatogenesis and oogenesis specific basic helix-loop-helix 1
别名 C9orf157, NOHLH, SOHLH1, TEB2
计算分子量 328 aa, 34 kDa
GenBank蛋白编号BC031861
基因名称 SOHLH1
Gene ID (NCBI) 402381
RRIDAB_2878434
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ5JUK2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Spermatogenesis‐and oogenesis‐specific helix‐loop‐helix transcription factor 1 (SOHLH1) is a gonad‐specific transcription factor that belongs to the basic‐helix‐loop‐helix (bHLH) transcription factor family. SOHLH1 has a profound effect on the development of germ cells, from SSC self-maintenance to spermatogonium differentiation.

实验方案

Product Specific Protocols
IHC protocol for SOHLH1 antibody 17753-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB, IF

Hum Mol Genet

The mutation c.346-1G > a in SOHLH1 impairs sperm production in the homozygous but not in the heterozygous condition.

Authors - Mohan Liu
View Article