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验证数据展示

  • WB analysis using 14316-1-AP (same clone as 14316-1-PBS)

    WB analysis using 14316-1-AP (same clone as 14316-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 14316-1-AP (SOD3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14316-1-PBS in a different storage buffer formulation.

  • IP experiment of human placenta using 14316-1-AP (same clone as 14316-1-PBS)

    IP experiment of human placenta using 14316-1-AP (same clone as 14316-1-PBS)

    IP result of anti-SOD3 (IP:14316-1-AP, 4ug; Detection:14316-1-AP 1:1000) with human placenta tissue lysate 2800ug. This data was developed using the same antibody clone with 14316-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

SOD3 Polyclonal antibody, PBS Only

SOD3 Polyclonal Antibody for WB, IP, Indirect ELISA
Cat No. 14316-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IP, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

14316-1-PBS targets SOD3 in WB, IP, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IP, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag5556

Product name: Recombinant human SOD3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-240 aa of BC014418

Sequence: MLALLCSCLLLAAGASDAWTGEDSAEPNSDSAEWIRDMYAKVTEIWQEVMQRRDDDGTLHAACQVQPSATLDAAQPRVTGVVLFRQLAPRAKLDAFFALEGFPTEPNSSSRAIHVHQFGDLSQGCESTGPHYNPLAVPHPQHPGDFGNFAVRDGSLWRYRAGLAASLAGPHSIVGRAVVVHAGEDDLGRGGNQASVENGNAGRRLACCVVGVCGPGLWERQAREHSERKKRRRESECKAA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 superoxide dismutase 3, extracellular
别名
计算分子量 26 kDa
观测分子量 25-33 kDa
GenBank蛋白编号BC014418
基因名称 SOD3
Gene ID (NCBI) 6649
RRIDAB_10898170
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP08294
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SOD is located intracellular (SOD-1; cytosolic, SOD-2; mitochondrial matrix) and extracellular (SOD-3), and catalyzes the dismutation of superoxide anion to H2O2. SOD3, or EC-SOD, is the most recently characterized SOD, exists as a copper and zinc-containing tetramer, and is synthesized containing a signal peptide that directs this enzyme exclusively to extracellular spaces. SOD-3 is a Mn-containing tetrameric protein with a subunit Mr of 24-35 kDa. The activity of EC-SOD is dependent on its ability to form a tetramer of 135 kDa.

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