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SOD3 Polyclonal antibody

SOD3 Polyclonal Antibody for WB, IP, ELISA
Cat No. 14316-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human and More (2)

应用

WB, IP, ELISA

EC SOD, Extracellular superoxide dismutase, SOD3, Superoxide Dismutase 3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存


经过测试的应用

Positive WB detected inhuman urine sample, human heart tissue, human urine, human placenta tissue
Positive IP detected inhuman placenta tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14316-1-AP targets SOD3 in WB, IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IP, ELISA Application Description
文献引用应用WB
经测试反应性 human
文献引用反应性human, mouse, rat
免疫原

CatNo: Ag5556

Product name: Recombinant human SOD3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-240 aa of BC014418

Sequence: MLALLCSCLLLAAGASDAWTGEDSAEPNSDSAEWIRDMYAKVTEIWQEVMQRRDDDGTLHAACQVQPSATLDAAQPRVTGVVLFRQLAPRAKLDAFFALEGFPTEPNSSSRAIHVHQFGDLSQGCESTGPHYNPLAVPHPQHPGDFGNFAVRDGSLWRYRAGLAASLAGPHSIVGRAVVVHAGEDDLGRGGNQASVENGNAGRRLACCVVGVCGPGLWERQAREHSERKKRRRESECKAA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 superoxide dismutase 3, extracellular
别名 EC SOD, Extracellular superoxide dismutase, SOD3, Superoxide Dismutase 3
计算分子量 26 kDa
观测分子量 25-33 kDa
GenBank蛋白编号BC014418
基因名称 SOD3
Gene ID (NCBI) 6649
RRIDAB_10898170
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP08294
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SOD is located intracellular (SOD-1; cytosolic, SOD-2; mitochondrial matrix) and extracellular (SOD-3), and catalyzes the dismutation of superoxide anion to H2O2. SOD3, or EC-SOD, is the most recently characterized SOD, exists as a copper and zinc-containing tetramer, and is synthesized containing a signal peptide that directs this enzyme exclusively to extracellular spaces. SOD-3 is a Mn-containing tetrameric protein with a subunit Mr of 24-35 kDa. The activity of EC-SOD is dependent on its ability to form a tetramer of 135 kDa.

实验方案

Product Specific Protocols
WB protocol for SOD3 antibody 14316-1-APDownload protocol
IP protocol for SOD3 antibody 14316-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouse,humanWB

Br J Pharmacol

Inhibition of DNA methylation derepresses PPARγ and attenuates pulmonary fibrosis.

Authors - Ai Wei
mouseWB

J Proteome Res

Sex-Specific Alterations in Placental Proteomics Induced by Intrauterine Hyperglycemia

Authors - Zhuo-Ran Ren
humanWB

Transl Pediatr

Identification and evaluation of fructose-bisphosphate aldolase B as a potential diagnostic biomarker in choledochal cysts patients: a quantitative proteomic analysis.

Authors - Gedong Ming
WB

Sci Rep

The double inhibition of PDK1 and STAT3-Y705 prevents liver metastasis in colorectal cancer.

Authors - Wenjuan Qin
human,ratWB

Toxicol Appl Pharmacol

(-)-Epicatechin ameliorates cigarette smoke-induced lung inflammation via inhibiting ROS/NLRP3 inflammasome pathway in rats with COPD.

Authors - Xue Tian
humanWB

Clin Immunol

Oxidative stress is involved in immunosuppression and macrophage regulation in glioblastoma

Authors - Xisong Liang
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