SOD3 Polyclonal antibody
SOD3 Polyclonal Antibody for IP, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (2)
Applications
IP, WB,ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | human urine sample, human heart tissue, human urine, human placenta tissue |
Positive IP detected in | human placenta tissue |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:4000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB |
Sample-dependent, check data in validation data gallery |
发表文章中的应用
WB | See 3 publications below |
产品信息
14316-1-AP targets SOD3 in WB, IP, ELISA applications and shows reactivity with human samples.
Tested Applications | IP, WB,ELISA |
Cited Applications | WB |
Tested Reactivity | human |
Cited Reactivity | human, mouse, rat |
Immunogen | SOD3 fusion protein Ag5556 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | superoxide dismutase 3, extracellular |
Synonyms | EC SOD, Extracellular superoxide dismutase, SOD3, Superoxide Dismutase 3 |
Calculated molecular weight | 26 kDa |
Observed molecular weight | 25-33 kDa |
GenBank accession number | BC014418 |
Gene symbol | SOD3 |
Uniprot | P08294 |
Gene ID (NCBI) | 6649 |
RRID | AB_10898170 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SOD is located intracellular (SOD-1; cytosolic, SOD-2; mitochondrial matrix) and extracellular (SOD-3), and catalyzes the dismutation of superoxide anion to H2O2.SOD3, or EC-SOD, is the most recently characterized SOD, exists as a copper and zinc-containing tetramer, and is synthesized containing a signal peptide that directs this enzyme exclusively to extracellular spaces. SOD-3 is a Mn-containing tetrameric protein with a subunit Mr of 24-35 kDa. The activity of EC-SOD is dependent on its ability to form a tetramer of 135 kDa.
实验方案
Product Specific Protocols | |
---|---|
WB protocol for SOD3 antibody 14316-1-AP | Download protocol |
IP protocol for SOD3 antibody 14316-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Br J Pharmacol Inhibition of DNA methylation derepresses PPARγ and attenuates pulmonary fibrosis. | ||
Toxicol Appl Pharmacol (-)-Epicatechin ameliorates cigarette smoke-induced lung inflammation via inhibiting ROS/NLRP3 inflammasome pathway in rats with COPD. | ||
Transl Pediatr Identification and evaluation of fructose-bisphosphate aldolase B as a potential diagnostic biomarker in choledochal cysts patients: a quantitative proteomic analysis. |