CoraLite®594-conjugated SOCS3 Monoclonal antibody
SOCS3 Monoclonal Antibody for FC (Intra), IF
Host / Isotype
Mouse / IgG1
Reactivity
Human, mouse
Applications
FC (Intra), IF
Conjugate
CoraLite®594 Fluorescent Dye
CloneNo.
1G2B12
验证数据展示
经过测试的应用
| Positive IF detected in | mouse heart tissue |
| Positive FC detected in | Jurkat cells |
For other applications, we recommend the unconjugated version of this antibody, 66797-1-Ig
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL594-66797 targets SOCS3 in IF, FC (Intra) applications and shows reactivity with Human, mouse samples.
| Tested Applications | FC (Intra), IF |
| Tested Reactivity | Human, mouse |
| Immunogen | SOCS3 fusion protein Ag5386 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | suppressor of cytokine signaling 3 |
| Synonyms | ATOD4, CIS 3, CIS3, Cish3, SOCS 3, SOCS3, SOCS-3, SSI 3, SSI3, STAT induced STAT inhibitor 3 |
| Calculated Molecular Weight | 25 kDa |
| Observed Molecular Weight | 25 kDa |
| GenBank Accession Number | BC060858 |
| Gene Symbol | SOCS3 |
| Gene ID (NCBI) | 9021 |
| Conjugate | CoraLite®594 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 588 nm / 604 nm |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | O14543 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Suppressor of cytokine signaling 3 (SOCS3) is a member of SOCS family that these proteins induced to attenuate cytokine signal transduction in response to signals from a diverse range of cytokines and growth factors. SOCS3 is a negative regulatory protein that inhibits signaling induced by IL-6 via preventing JAK mediated activation of STAT3. However SOCS3 can positively regulate the ERK-MAPK pathway, inhibit the NF-kB pathway (PMID:26429311,25035930). In addition, SOCS3 brings a great impact on immune responses by inhibiting signaling stimulus, such as LPS, type I and type II IFNs, and IL-12. SOCS3 is widely expressed in heart, placenta, skeletal muscle, peripheral blood leukocytes, fetal and adult lung, and fetal liver and kidney (PMID: 22961088). Dysregulation of SOCS3 functions can cause a variety of diseases, including allergy, autoimmune diseases, inflammation and cancer (PMID:26429311).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL594 SOCS3 antibody CL594-66797 | Download protocol |
| FC protocol for CL594 SOCS3 antibody CL594-66797 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
