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验证数据展示

  • IHC staining of human breast cancer using 66797-1-Ig (same clone as 66797-1-PBS)

    IHC staining of human breast cancer using 66797-1-Ig (same clone as 66797-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66797-1-Ig (SOCS3 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66797-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 66797-1-Ig (same clone as 66797-1-PBS)

    IHC staining of human breast cancer using 66797-1-Ig (same clone as 66797-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66797-1-Ig (SOCS3 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66797-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse heart using 66797-1-Ig (same clone as 66797-1-PBS)

    IF Staining of mouse heart using 66797-1-Ig (same clone as 66797-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using SOCS3 antibody (66797-1-Ig, Clone: 1G2B12 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66797-1-PBS in a different storage buffer formulation.

  • WB analysis using 66797-1-Ig (same clone as 66797-1-PBS)

    WB analysis using 66797-1-Ig (same clone as 66797-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66797-1-Ig (SOCS3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66797-1-PBS in a different storage buffer formulation.

SOCS3 Monoclonal antibody, PBS Only

SOCS3 Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 66797-1-PBS

产品说明书

CloneNo. 1G2B12

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse

应用

WB, IHC, IF-P, Indirect ELISA

别名

1G2B12, ATOD4, CIS3, CIS-3, Cytokine-inducible SH2 protein 3

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

66797-1-PBS targets SOCS3 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF-P, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag5386

Product name: Recombinant human SOCS3 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-225 aa of BC060858

Sequence: MVTHSKFPAAGMSRPLDTSLRLKTFSSKSEYQLVVNAVRKLQESGFYWSAVTGGEANLLLSAEPAGTFLIRDSSDQRHFFTLSVKTQSGTKNLRIQCEGGSFSLQSDPRSTQPVPRFDCVLKLVHHYMPPPGAPSFPSPPTEPSSEVPEQPSAQPLPGSPPRRAYYIYSGGEKIPLVLSRPLSSNVATLQHLCRKTVNGHLDSYEKVTQLPGPIREFLDQYDAPL

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 suppressor of cytokine signaling 3
别名 1G2B12, ATOD4, CIS3, CIS-3, Cytokine-inducible SH2 protein 3
计算分子量 25 kDa
观测分子量 25 kDa
GenBank蛋白编号BC060858
基因名称 SOCS3
Gene ID (NCBI) 9021
RRIDAB_2882141
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDO14543
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Suppressor of cytokine signaling 3 (SOCS3) is a member of SOCS family that these proteins induced to attenuate cytokine signal transduction in response to signals from a diverse range of cytokines and growth factors. SOCS3 is a negative regulatory protein that inhibits signaling induced by IL-6 via preventing JAK mediated activation of STAT3. However SOCS3 can positively regulate the ERK-MAPK pathway, inhibit the NF-kB pathway (PMID:26429311,25035930). In addition, SOCS3 brings a great impact on immune responses by inhibiting signaling stimulus, such as LPS, type I and type II IFNs, and IL-12. SOCS3 is widely expressed in heart, placenta, skeletal muscle, peripheral blood leukocytes, fetal and adult lung, and fetal liver and kidney (PMID: 22961088). Dysregulation of SOCS3 functions can cause a variety of diseases, including allergy, autoimmune diseases, inflammation and cancer (PMID:26429311).

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