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验证数据展示

  • WB analysis of HepG2 using 86446-1-RR

    WB analysis of HepG2 using 86446-1-RR

    HepG2 cells were subjected to SDS PAGE followed by western blot with 86446-1-RR (SNX29 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis of HepG2 using 86446-1-RR

    WB analysis of HepG2 using 86446-1-RR

    WB result of SNX29 antibody (86446-1-RR; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SNX29 transfected HepG2 cells.

  • IF Staining of HeLa using 86446-1-RR

    IF Staining of HeLa using 86446-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SNX29 antibody (86446-1-RR, Clone: 251222A2 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 86446-1-RR

    Affinity and Kinetic Characterization of 86446-1-RR

    Biolayer interferometry (BLl) kinetic assays of 86446-1-RR against Human SNX29 were performed. The affinity constant is 11.7 nM.

  • Featured Product
  • KD/KO Validated

SNX29 Recombinant monoclonal antibody

SNX29 Uni-rAb® Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 86446-1-RR

产品说明书

CloneNo. 251222A2

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

RUN domain-containing protein 2A, RUNDC2A, Sorting nexin-29

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHepG2 cells
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

86446-1-RR targets SNX29 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag32618

Product name: Recombinant human SNX29 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-150 aa of XM_011522738

Sequence: MSGSQNNDKRQFLLERLLDAVKQCQIRFGGRKEIASDSDSRVTCLCAQFEAVLQHGLKRSRGLALTAAAIKQAAGFASKTETEPVFWYYVKEVLNKHELQRFYSLRHIASDVGRGRAWLRCALNEHSLERYLHMLLADRCRLSTFYEDWS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 sorting nexin 29
别名 RUN domain-containing protein 2A, RUNDC2A, Sorting nexin-29
计算分子量91kd
观测分子量90 kDa
GenBank蛋白编号XM_011522738
基因名称 SNX29
Gene ID (NCBI) 92017
RRIDAB_3744888
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ8TEQ0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Sorting nexins are a diverse group of cytoplasmic and membrane-associated proteins classified by the presence of a phospholipid-binding motif PX domain (PMID:12461558). They are involved in endocytosis and protein trafficking. SNX29 (Sorting nexin 29) is associated with bipolar disorder (BPD) and major depressive disorder (MDD) in the Han population (PMID: 33143498). The expression of SNX29 is significantly upregulated in most tumor tissues, and its expression is associated with the progression, immune infiltration and drug sensitivity of various cancers (PMID: 36829159).

实验方案

Product Specific Protocols
IF protocol for SNX29 antibody 86446-1-RRDownload protocol
WB protocol for SNX29 antibody 86446-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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