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验证数据展示

  • WB analysis of HepG2 using 86446-1-RR (same clone as 86446-1-PBS)

    WB analysis of HepG2 using 86446-1-RR (same clone as 86446-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 86446-1-RR (SNX29 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86446-1-PBS in a different storage buffer formulation.

  • WB analysis of HepG2 using 86446-1-RR (same clone as 86446-1-PBS)

    WB analysis of HepG2 using 86446-1-RR (same clone as 86446-1-PBS)

    WB result of SNX29 antibody (86446-1-RR; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SNX29 transfected HepG2 cells. This data was developed using the same antibody clone with 86446-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 86446-1-RR (same clone as 86446-1-PBS)

    IF Staining of HeLa using 86446-1-RR (same clone as 86446-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SNX29 antibody (86446-1-RR, Clone: 251222A2 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86446-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 86446-1-RR

    Affinity and Kinetic Characterization of 86446-1-RR

    Biolayer interferometry (BLl) kinetic assays of 86446-1-RR against Human SNX29 were performed. The affinity constant is 11.7 nM.

  • Featured Product
  • KD/KO Validated

SNX29 Recombinant monoclonal antibody, PBS Only

SNX29 Uni-rAb® Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 86446-1-PBS

产品说明书

CloneNo. 251222A2

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, Indirect ELISA

别名

RUN domain-containing protein 2A, RUNDC2A, Sorting nexin-29

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

86446-1-PBS targets SNX29 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag32618

Product name: Recombinant human SNX29 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-150 aa of XM_011522738

Sequence: MSGSQNNDKRQFLLERLLDAVKQCQIRFGGRKEIASDSDSRVTCLCAQFEAVLQHGLKRSRGLALTAAAIKQAAGFASKTETEPVFWYYVKEVLNKHELQRFYSLRHIASDVGRGRAWLRCALNEHSLERYLHMLLADRCRLSTFYEDWS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 sorting nexin 29
别名 RUN domain-containing protein 2A, RUNDC2A, Sorting nexin-29
计算分子量91kd
观测分子量90 kDa
GenBank蛋白编号XM_011522738
基因名称 SNX29
Gene ID (NCBI) 92017
RRIDAB_3744888
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ8TEQ0
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Sorting nexins are a diverse group of cytoplasmic and membrane-associated proteins classified by the presence of a phospholipid-binding motif PX domain (PMID:12461558). They are involved in endocytosis and protein trafficking. SNX29 (Sorting nexin 29) is associated with bipolar disorder (BPD) and major depressive disorder (MDD) in the Han population (PMID: 33143498). The expression of SNX29 is significantly upregulated in most tumor tissues, and its expression is associated with the progression, immune infiltration and drug sensitivity of various cancers (PMID: 36829159).

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