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验证数据展示

  • WB analysis using 68305-1-Ig

    WB analysis using 68305-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68305-1-Ig (SNX18 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of A431 using 68305-1-Ig

    IF Staining of A431 using 68305-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using SNX18 antibody (68305-1-Ig, Clone: 2E7D6 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

SNX18 Monoclonal antibody

SNX18 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 68305-1-Ig

产品说明书

CloneNo. 2E7D6

宿主/亚型

Mouse / IgG1

种属反应性

Human, Mouse

应用

WB, IF/ICC, ELISA

别名

FLJ11997, FLJ32560, FLJ61062, SH3PX2, SH3PXD3B, SNAG1, SNX18, sorting nexin 18

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive WB detected inHCT 116 cells, NIH/3T3 cells, A549 cells, HeLa cells, LNCaP cells, HEK-293 cells, Jurkat cells, K-562 cells
Positive IF/ICC detected inA431 cells
Planning an IHC experiment? We recommend our IHCeasy SNX18 Ready-To-Use IHC Kit. SNX18 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68305-1-Ig targets SNX18 in WB, IF/ICC, ELISA applications and shows reactivity with Human, Mouse samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 Human, Mouse
免疫原

CatNo: Ag17388

Product name: Recombinant human SNX18 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 55-284 aa of BC060791

Sequence: YVQVIRAPEPGPAGDGGPGAPARYANVPPGGFEPLPVAPPASFKPPPDAFQALLQPQQAPPPSTFQPPGAGFPYGGGALQPSPQQLYGGYQASQGSDDDWDDEWDDSSTVADEPGALGSGAYPDLDGSSSAGVGAAGRYRLSTRSDLSLGSRGGSVPPQHHPSGPKSSATVSRNLNRFSTFVKSGGEAFVLGEASGFVKDGDKLCVVLGPYGPEWQENPYPFQCTIDDPT

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 sorting nexin 18
别名 FLJ11997, FLJ32560, FLJ61062, SH3PX2, SH3PXD3B, SNAG1, SNX18, sorting nexin 18
计算分子量 628 aa, 69 kDa
观测分子量 67-70 kDa
GenBank蛋白编号BC060791
基因名称 SNX18
Gene ID (NCBI) 112574
RRIDAB_2935384
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ96RF0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SNXs (sorting nexins) are a diverse group of cytoplasmic and membrane-associated proteins that are classified by the presence of a phospholipid-binding motif-the PX domain (PMID:12461558). SNXs are involved in endocytosis and protein trafficking. SNX18, together with SNX9 and SNX33, constitutes a separate SNX9 subfamily which is required for mitosis through endocytosis processes (PMID: 22718350, 20427313). SNX18 acts as a positive regulator of autophagy, and regulates ATG9A trafficking from recycling endosomes (PMID: 24113029, 29437695).

实验方案

Product Specific Protocols
IF protocol for SNX18 antibody 68305-1-IgDownload protocol
WB protocol for SNX18 antibody 68305-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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