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SNX15 Monoclonal antibody

SNX15 Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

1A10B7

Cat No : 68239-1-Ig

Print datasheet

Synonyms

HSAF001435, SNX15, sorting nexin 15

验证数据展示

  • WB analysis using 68239-1-Ig

    WB analysis using 68239-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68239-1-Ig (SNX15 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • IHC staining of human kidney using 68239-1-Ig

    IHC staining of human kidney using 68239-1-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 68239-1-Ig (SNX15 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human kidney using 68239-1-Ig

    IHC staining of human kidney using 68239-1-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 68239-1-Ig (SNX15 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inLNCaP cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68239-1-Ig targets SNX15 in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Applications IHC, WB, ELISA
Tested Reactivity Human
Immunogen SNX15 fusion protein Ag8839 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name sorting nexin 15
Synonyms HSAF001435, SNX15, sorting nexin 15
Calculated Molecular Weight 342 aa, 38 kDa
Observed Molecular Weight 40-50 kDa
GenBank Accession NumberBC009897
Gene Symbol SNX15
Gene ID (NCBI) 29907
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9NRS6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SNX15 belongs to the sorting nexin family and is involved in several stages of intracellular trafficking. Overexpression of SNX15 results in a decrease in the processing of insulin and hepatocyte growth factor receptors to their mature subunits (PMID: 11085978). SNX15 is widely expressed, with the highest expression in skeletal muscle, heart, brain, kidney, spleen, thymus, and small intestine.

实验方案

Product Specific Protocols
WB protocol for SNX15 antibody 68239-1-IgDownload protocol
IHC protocol for SNX15 antibody 68239-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols