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验证数据展示

  • WB analysis of HepG2 using 25926-1-AP

    WB analysis of HepG2 using 25926-1-AP

    WB result of SNW1 antibody (25926-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SNW1 transfected HepG2 cells.

  • IHC staining of human prostate cancer using 25926-1-AP

    IHC staining of human prostate cancer using 25926-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 25926-1-AP (SNW1 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HepG2 using 25926-1-AP

    IF Staining of HepG2 using 25926-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SNW1 antibody (25926-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • IHC staining of human prostate cancer using 25926-1-AP

    IHC staining of human prostate cancer using 25926-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 25926-1-AP (SNW1 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of MCF-7 using 25926-1-AP

    WB analysis of MCF-7 using 25926-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 25926-1-AP (SNW1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 25926-1-AP

    IF Staining of HepG2 using 25926-1-AP

    Immunofluorescent analysis of HepG2 cells using 25926-1-AP (SNW1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

  • Featured Product
  • KD/KO验证

SNW1 Polyclonal antibody

SNW1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 25926-1-AP

产品说明书

引用文献(4)

宿主/亚型

Rabbit / IgG

种属反应性

human and More (1)

应用

WB, IHC, IF/ICC, ELISA

别名

NCOA 62, SKIP, SKIIP, Ski-interacting protein, Nuclear receptor coactivator NCoA-62

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inMCF-7 cells, HepG2 cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:150-1:600
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25926-1-AP targets SNW1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB
经测试反应性 human
文献引用反应性human, mouse
免疫原

CatNo: Ag23106

Product name: Recombinant human SNW1 protein

Source: e coli.-derived, PET30a

Tag: 6*His

Domain: 188-536 aa of BC108903

Sequence: FNSGAKQRVIRMVEMQKDPMEPPRFKINKKIPRGPPSPPAPVMHSPSRKMTVKEQQEWKIPPCISNWKNAKGYTIPLDKRLAADGRGLQTVHINENFAKLAEALYIADRKAREAVEMRAQVERKMAQKEKEKHEEKLREMAQKARERRAGIKTHVEKEDGEARERDEIRHDRRKERQHDRNLSRAAPDKRSKLQRNENRDISEVIALGVPNPRTSNEVQYDQRLFNQSKGMDSGFAGGEDEIYNVYDQAWRGGKDMAQSIYRPSKNLDKDMYGDDLEARIKTNRFVPDKEFSGSDRRQRGREGPVQFEEDPFGLDKFLEEAKQHGGSKRPSDSSRPKEHEHEGKKRRKE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 SNW domain containing 1
别名 NCOA 62, SKIP, SKIIP, Ski-interacting protein, Nuclear receptor coactivator NCoA-62
计算分子量 536 aa, 61 kDa
观测分子量 62-69 kDa
GenBank蛋白编号BC108903
基因名称 SNW1
Gene ID (NCBI) 22938
RRIDAB_2880298
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ13573
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for SNW1 antibody 25926-1-APDownload protocol
IHC protocol for SNW1 antibody 25926-1-APDownload protocol
WB protocol for SNW1 antibody 25926-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nucleic Acids Res

XAB2 depletion induces intron retention in POLR2A to impair global transcription and promote cellular senescence.

Authors - Shuai Hou
View Article
humanWB

FEBS Lett

Split-BioID: a proximity biotinylation assay for dimerization-dependent protein interactions.

Authors - Sofie De Munter
View Article
humanWB

Microbes Infect

SNW1 interacts with IKKγ to positively regulate antiviral innate immune responses against influenza A virus infection.

Authors - Qiao Zhang
  • KD Validated
View Article
mouseWB

J Ovarian Res

Alteration of the N6-methyladenosine methylation landscape in a mouse model of polycystic ovary syndrome

Authors - Lingxiao Zou
View Article
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