验证数据展示
经过测试的应用
| Positive WB detected in | MCF-7 cells, HepG2 cells |
| Positive IHC detected in | human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:150-1:600 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 4 publications below |
产品信息
25926-1-AP targets SNW1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse |
| 免疫原 | SNW1 fusion protein Ag23106 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | SNW domain containing 1 |
| 别名 | NCOA 62, SKIP, SKIIP, Ski-interacting protein, Nuclear receptor coactivator NCoA-62 |
| 计算分子量 | 536 aa, 61 kDa |
| 观测分子量 | 62-69 kDa |
| GenBank蛋白编号 | BC108903 |
| 基因名称 | SNW1 |
| Gene ID (NCBI) | 22938 |
| RRID | AB_2880298 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q13573 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SNW1 antibody 25926-1-AP | Download protocol |
| IHC protocol for SNW1 antibody 25926-1-AP | Download protocol |
| IF protocol for SNW1 antibody 25926-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nucleic Acids Res XAB2 depletion induces intron retention in POLR2A to impair global transcription and promote cellular senescence. | ||
FEBS Lett Split-BioID: a proximity biotinylation assay for dimerization-dependent protein interactions. | ||
Microbes Infect SNW1 interacts with IKKγ to positively regulate antiviral innate immune responses against influenza A virus infection.
| ||
J Ovarian Res Alteration of the N6-methyladenosine methylation landscape in a mouse model of polycystic ovary syndrome |
