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SNRPF Polyclonal antibody

SNRPF Polyclonal Antibody for FC, IF/ICC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC, IF/ICC, IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 14977-1-AP

Print datasheet

Synonyms

PBSCF, Sm F, Sm protein F, SMF, snRNP F, SNRPF

验证数据展示

  • WB analysis of human heart using 14977-1-AP

    WB analysis of human heart using 14977-1-AP

    human heart tissue were subjected to SDS PAGE followed by western blot with 14977-1-AP (SNRPF antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 14977-1-AP

    IHC staining of human breast cancer using 14977-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 14977-1-AP (SNRPF antibody) at dilution of 1:100 (under 40x lens).

    Ready-to-use IHC kits available with SNRPF antibody included
  • IF Staining of HeLa using 14977-1-AP

    IF Staining of HeLa using 14977-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 14977-1-AP (SNRPF antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • FC experiment of MCF-7 using 14977-1-AP

    FC experiment of MCF-7 using 14977-1-AP

    1X10^6 MCF-7 cells were stained with 0.2ug SNRPF antibody (14977-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

经过测试的应用

Positive WB detected inhuman heart tissue, HeLa cells, HepG2 cells, MCF-7 cells, mouse kidney tissue, mouse liver tissue
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Positive FC detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy SNRPF Ready-To-Use IHC Kit. SNRPF primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14977-1-AP targets SNRPF in WB, IF, FC, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications FC, IF/ICC, IHC, WB, ELISA
Tested Reactivity human, mouse, rat
Immunogen SNRPF fusion protein Ag6889 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name small nuclear ribonucleoprotein polypeptide F
Synonyms PBSCF, Sm F, Sm protein F, SMF, snRNP F, SNRPF
Calculated Molecular Weight 10 kDa
Observed Molecular Weight 10 kDa
GenBank Accession NumberBC002505
Gene Symbol SNRPF
Gene ID (NCBI) 6636
RRIDAB_2302166
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP62306
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The 4 major small nuclear ribonucleoprotein particles (snRNPs), U1, U2, U4/U6, and U5, are essential components of the eukaryotic pre-mRNA splicing machinery. These snRNPs share 8 proteins which form the snRNP structural core: B/B-prime (SNRPB), D1 (SNRPD1), D2 (SNRPD2), D3 (SNRPD3), E (SNRPE), F (SNRPF), and G (SNRPG). These proteins have a role in the biogenesis of the snRNPs.

实验方案

Product Specific Protocols
WB protocol for SNRPF antibody 14977-1-APDownload protocol
IHC protocol for SNRPF antibody 14977-1-APDownload protocol
IF protocol for SNRPF antibody 14977-1-APDownload protocol
FC protocol for SNRPF antibody 14977-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols