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验证数据展示

  • WB analysis of human heart using 14977-1-AP

    WB analysis of human heart using 14977-1-AP

    human heart tissue were subjected to SDS PAGE followed by western blot with 14977-1-AP (SNRPF antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 14977-1-AP

    IHC staining of human breast cancer using 14977-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 14977-1-AP (SNRPF antibody) at dilution of 1:100 (under 40x lens).

    Ready-to-use IHC kits available with SNRPF antibody included
  • IF Staining of HeLa using 14977-1-AP

    IF Staining of HeLa using 14977-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 14977-1-AP (SNRPF antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

SNRPF Polyclonal antibody

SNRPF Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 14977-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

snRNP F, Sm-F, SMF, Sm protein F, Sm F

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inhuman heart tissue, HeLa cells, HepG2 cells, MCF-7 cells, mouse kidney tissue, mouse liver tissue
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy SNRPF Ready-To-Use IHC Kit. SNRPF primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14977-1-AP targets SNRPF in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 SNRPF fusion protein Ag6889 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 small nuclear ribonucleoprotein polypeptide F
别名 snRNP F, Sm-F, SMF, Sm protein F, Sm F
计算分子量 10 kDa
观测分子量 10 kDa
GenBank蛋白编号BC002505
基因名称 SNRPF
Gene ID (NCBI) 6636
RRIDAB_2302166
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP62306
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The 4 major small nuclear ribonucleoprotein particles (snRNPs), U1, U2, U4/U6, and U5, are essential components of the eukaryotic pre-mRNA splicing machinery. These snRNPs share 8 proteins which form the snRNP structural core: B/B-prime (SNRPB), D1 (SNRPD1), D2 (SNRPD2), D3 (SNRPD3), E (SNRPE), F (SNRPF), and G (SNRPG). These proteins have a role in the biogenesis of the snRNPs.

实验方案

Product Specific Protocols
WB protocol for SNRPF antibody 14977-1-APDownload protocol
IHC protocol for SNRPF antibody 14977-1-APDownload protocol
IF protocol for SNRPF antibody 14977-1-APDownload protocol
FC protocol for SNRPF antibody 14977-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols