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验证数据展示

  • WB analysis of A2780 using 14789-1-AP

    WB analysis of A2780 using 14789-1-AP

    A2780 cells were subjected to SDS PAGE followed by western blot with 14789-1-AP (SNRPD2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human skin using 14789-1-AP

    IHC staining of human skin using 14789-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 14789-1-AP (SNRPD2 Antibody) at dilution of 1:50.

    Ready-to-use IHC kits available with SNRPD2 antibody included

SNRPD2 Polyclonal antibody

SNRPD2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 14789-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

Sm D2, SMD2, snRNP core protein D2, SNRPD2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA2780 cells, A549 cells, HL-60 cells
Positive IHC detected inhuman skin tissue, human lung cancer tissue, human lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy SNRPD2 Ready-To-Use IHC Kit. SNRPD2 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2400
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

14789-1-AP targets SNRPD2 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human
文献引用反应性human
免疫原 SNRPD2 fusion protein Ag6443 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 small nuclear ribonucleoprotein D2 polypeptide 16.5kDa
别名 Sm D2, SMD2, snRNP core protein D2, SNRPD2
计算分子量 14 kDa
观测分子量 14-16 kDa
GenBank蛋白编号BC000486
基因名称 SNRPD2
Gene ID (NCBI) 6633
RRIDAB_10898359
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP62316
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Systemic lupus erythematosus is characterized by antibodies to a variety of intracellular self-antigens, such as dsDNA and Sm, and these serve as hallmarks in the diagnosis of systemic autoimmune diseases. SNRPD2 is one of Sm protein, and is required for pre-mRNA splicing, snRNP biogenesis.

实验方案

Product Specific Protocols
WB protocol for SNRPD2 antibody 14789-1-APDownload protocol
IHC protocol for SNRPD2 antibody 14789-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

RNA Biol

Nuclear retention element recruits U1 snRNP components to restrain spliced lncRNAs in the nucleus.

Authors - Sikandar Azam
View Article