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验证数据展示

  • Cytometric bead array sample test of MP51752-1

    Cytometric bead array sample test of MP51752-1

    Cytometric bead array sample test of MP51752-1, SNRPB2 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68095-2-PBS. Detection antibody: 68095-1-PBS.

  • WB analysis using 68095-1-Ig (same clone as 68095-1-PBS)

    WB analysis using 68095-1-Ig (same clone as 68095-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68095-1-Ig (SNRPB2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 68095-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 68095-1-Ig (same clone as 68095-1-PBS)

    FC experiment of HeLa using 68095-1-Ig (same clone as 68095-1-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.8 ug SNRPB2 Monoclonal antibody (68095-1-Ig, Clone:1D11E9) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.8 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 68095-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP51752-1

    Cytometric bead array standard curve of MP51752-1

    Cytometric bead array standard curve of MP51752-1, SNRPB2 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68095-2-PBS. Detection antibody: 68095-1-PBS. Standard:Ag5540. Range: 0.391-50 ng/mL.

SNRPB2 Monoclonal antibody, PBS Only (Detector)

SNRPB2 Monoclonal Antibody for WB, FC (Intra), Cytometric bead array, Indirect ELISA
Cat No. 68095-1-PBS

产品说明书

CloneNo. 1D11E9

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, FC (Intra), Cytometric bead array, Indirect ELISA

别名

1D11E9, U2 small nuclear ribonucleoprotein B'', U2 snRNP B, U2 snRNP B''

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

68095-1-PBS targets SNRPB2 as part of a matched antibody pair:

MP51752-1: 68095-2-PBS capture and 68095-1-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, FC (Intra), Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag5540

Product name: Recombinant human SNRPB2 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-225 aa of BC036737

Sequence: MDIRPNHTIYINNMNDKIKKEELKRSLYALFSQFGHVVDIVALKTMKMRGQAFVIFKELGSSTNALRQLQGFPFYGKPMRIQYAKTDSDIISKMRGTFADKEKKKEKKKAKTVEQTATTTNKKPGQGTPNSANTQGNSTPNPQVPDYPPNYILFLNNLPEETNEMMLSMLFNQFPGFKEVRLVPGRHDIAFVEFENDGQAGAARDALQGFKITPSHAMKITYAKK

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 small nuclear ribonucleoprotein polypeptide B''
别名 1D11E9, U2 small nuclear ribonucleoprotein B'', U2 snRNP B, U2 snRNP B''
计算分子量 225 aa, 26, 5 kDa
观测分子量25-31 kDa
GenBank蛋白编号BC036737
基因名称 SNRPB2
Gene ID (NCBI) 6629
RRIDAB_2918832
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDP08579
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

U2 small nuclear ribonucleoprotein B''(SNRPB2) is part of the U2 RNP particle, which associated with the branch point of the lariat-shaped intron, in intermediate in the cascade of mRNA precursor splicing event. Only in presence of the U2A' protein, SNRPB2 binds stem loop IV of U2 snRNA.

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