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验证数据展示

  • WB analysis using 68095-1-Ig

    WB analysis using 68095-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68095-1-Ig (SNRPB2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

  • FC experiment of HeLa using 68095-1-Ig

    FC experiment of HeLa using 68095-1-Ig

    1x10^6 HeLa cells were intracellularly stained with 0.8 ug SNRPB2 Monoclonal antibody (68095-1-Ig, Clone:1D11E9) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.8 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

SNRPB2 Monoclonal antibody

SNRPB2 Monoclonal Antibody for WB, FC (Intra), ELISA
Cat No. 68095-1-Ig

产品说明书

CloneNo. 1D11E9

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, FC (Intra), ELISA

别名

U2 snRNP B'', U2 snRNP B, U2 small nuclear ribonucleoprotein B'', 1D11E9

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, Jurkat cells, K-562 cells
Positive FC (Intra) detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68095-1-Ig targets SNRPB2 in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag5540

Product name: Recombinant human SNRPB2 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-225 aa of BC036737

Sequence: MDIRPNHTIYINNMNDKIKKEELKRSLYALFSQFGHVVDIVALKTMKMRGQAFVIFKELGSSTNALRQLQGFPFYGKPMRIQYAKTDSDIISKMRGTFADKEKKKEKKKAKTVEQTATTTNKKPGQGTPNSANTQGNSTPNPQVPDYPPNYILFLNNLPEETNEMMLSMLFNQFPGFKEVRLVPGRHDIAFVEFENDGQAGAARDALQGFKITPSHAMKITYAKK

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 small nuclear ribonucleoprotein polypeptide B''
别名 U2 snRNP B'', U2 snRNP B, U2 small nuclear ribonucleoprotein B'', 1D11E9
计算分子量 225 aa, 26, 5 kDa
观测分子量25-31 kDa
GenBank蛋白编号BC036737
基因名称 SNRPB2
Gene ID (NCBI) 6629
RRIDAB_2918832
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDP08579
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

U2 small nuclear ribonucleoprotein B''(SNRPB2) is part of the U2 RNP particle, which associated with the branch point of the lariat-shaped intron, in intermediate in the cascade of mRNA precursor splicing event. Only in presence of the U2A' protein, SNRPB2 binds stem loop IV of U2 snRNA.

实验方案

Product Specific Protocols
FC protocol for SNRPB2 antibody 68095-1-IgDownload protocol
WB protocol for SNRPB2 antibody 68095-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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