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验证数据展示

  • IF Staining of HeLa using CL488-14950

    IF Staining of HeLa using CL488-14950

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 SNIP1 antibody (CL488-14950) at dilution of 1:200, CL594-Phalloidin (red).

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  • KD/KO验证

CoraLite® Plus 488-conjugated SNIP1 Polyclonal antibody

SNIP1 Polyclonal Antibody for IF/ICC
Cat No. CL488-14950

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IF/ICC

别名

RP3 423B22.3, SNIP1

缓冲液配方:  PBS, Proclin 300, BSA, Glycerol
PBS, Proclin 300, BSA, Glycerol
偶联物:  CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy SNIP1 Ready-To-Use IHC Kit. SNIP1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 14950-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-14950 targets SNIP1 in IF/ICC applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag6772

Product name: Recombinant human SNIP1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-396 aa of BC027040

Sequence: MKAVKSERERGSRRRHRDGDVVLPAGVVVKQERLSPEVAPPAHRRPDHSGGSPSPPTSEPARSGHRGNRARGVSRSPPKKKNKASGRRSKSPRSKRNRSPHHSTVKVKQEREDHPRRGREDRQHREPSEQEHRRARNSDRDRHRGHSHQRRTSNERPGSGQGQGRDRDTQNLQAQEEEREFYNARRREHRQRNDVGGGGSESQELVPRPGGNNKEKEVPAKEKPSFELSGALLEDTNTFRGVVIKYSEPPEARIPKKRWRLYPFKNDEVLPVMYIHRQSAYLLGRHRRIADIPIDHPSCSKQHAVFQYRLVEYTRADGTVGRRVKPYIIDLGSGNGTFLNNKRIEPQRYYELKEKDVLKFGFSSREYVLLHESSDTSEIDRKDDEDEEEEEEVSDS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 Smad nuclear interacting protein 1
别名 RP3 423B22.3, SNIP1
计算分子量 46 kDa
观测分子量 46-50 kDa
GenBank蛋白编号BC027040
基因名称 SNIP1
Gene ID (NCBI) 79753
RRIDAB_3672613
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8TAD8
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Smad nuclear-interacting protein 1 (SNIP1) is a novel interacting factor of the Smad protein family that is expressed in different cell types and is involved in cell proliferation, apoptosis, and differentiation.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 SNIP1 antibody CL488-14950Download protocol
Standard Protocols
Click here to view our Standard Protocols
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