验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, Jurkat cells, HT-29 cells, U2OS cells, BxPC-3 cells |
| Positive IF/ICC detected in | HeLa cells |
| Positive FC (Intra) detected in | U2OS cells |
| Positive FC detected in | U2OS cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:500-1:2000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| Flow Cytometry (FC) | FC : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
82962-1-RR targets SNIP1 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with Human samples.
| 经测试应用 | WB, IF/ICC, FC (Intra), ELISA Application Description |
| 经测试反应性 | Human |
| 免疫原 |
CatNo: Ag33543 Product name: Recombinant human SNIP1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-396 aa of BC027040 Sequence: MKAVKSERERGSRRRHRDGDVVLPAGVVVKQERLSPEVAPPAHRRPDHSGGSPSPPTSEPARSGHRGNRARGVSRSPPKKKNKASGRRSKSPRSKRNRSPHHSTVKVKQEREDHPRRGREDRQHREPSEQEHRRARNSDRDRHRGHSHQRRTSNERPGSGQGQGRDRDTQNLQAQEEEREFYNARRREHRQRNDVGGGGSESQELVPRPGGNNKEKEVPAKEKPSFELSGALLEDTNTFRGVVIKYSEPPEARIPKKRWRLYPFKNDEVLPVMYIHRQSAYLLGRHRRIADIPIDHPSCSKQHAVFQYRLVEYTRADGTVGRRVKPYIIDLGSGNGTFLNNKRIEPQRYYELKEKDVLKFGFSSREYVLLHESSDTSEIDRKDDEDEEEEEEVSDS* 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | Smad nuclear interacting protein 1 |
| 别名 | dJ423B22.2, FLJ12553, RP3 423B22.3, SNIP1 |
| 计算分子量 | 46 kDa |
| 观测分子量 | 46-50 kDa |
| GenBank蛋白编号 | BC027040 |
| 基因名称 | SNIP1 |
| Gene ID (NCBI) | 79753 |
| RRID | AB_3670708 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q8TAD8 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Smad nuclear-interacting protein 1 (SNIP1) is a novel interacting factor of the Smad protein family that is expressed in different cell types and is involved in cell proliferation, apoptosis, and differentiation.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for SNIP1 antibody 82962-1-RR | Download protocol |
| WB protocol for SNIP1 antibody 82962-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
