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验证数据展示

  • WB analysis using 82962-1-RR (same clone as 82962-1-PBS)

    WB analysis using 82962-1-RR (same clone as 82962-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82962-1-RR (SNIP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82962-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 82962-1-RR (same clone as 82962-1-PBS)

    IF Staining of HeLa using 82962-1-RR (same clone as 82962-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SNIP1 antibody (82962-1-RR, Clone: 230287D11 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 82962-1-PBS in a different storage buffer formulation.

  • FC experiment of U2OS using 82962-1-RR (same clone as 82962-1-PBS)

    FC experiment of U2OS using 82962-1-RR (same clone as 82962-1-PBS)

    1x10^6 U2OS cells were intracellularly stained with 0.25 ug SNIP1 Recombinant antibody (82962-1-RR, Clone:230287D11) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 82962-1-PBS in a different storage buffer formulation.

SNIP1 Recombinant monoclonal antibody, PBS Only

SNIP1 Uni-rAb® Recombinant Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 82962-1-PBS

产品说明书

CloneNo. 230287D11

宿主/亚型

Rabbit / IgG

种属反应性

Human

应用

WB, IF/ICC, FC (Intra), Indirect ELISA

别名

dJ423B22.2, FLJ12553, RP3 423B22.3, SNIP1

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy SNIP1 Ready-To-Use IHC Kit. SNIP1 primary antibody included.

产品信息

82962-1-PBS targets SNIP1 in WB, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with Human samples.

经测试应用 WB, IF/ICC, FC (Intra), Indirect ELISA Application Description
经测试反应性 Human
免疫原

CatNo: Ag33543

Product name: Recombinant human SNIP1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-396 aa of BC027040

Sequence: MKAVKSERERGSRRRHRDGDVVLPAGVVVKQERLSPEVAPPAHRRPDHSGGSPSPPTSEPARSGHRGNRARGVSRSPPKKKNKASGRRSKSPRSKRNRSPHHSTVKVKQEREDHPRRGREDRQHREPSEQEHRRARNSDRDRHRGHSHQRRTSNERPGSGQGQGRDRDTQNLQAQEEEREFYNARRREHRQRNDVGGGGSESQELVPRPGGNNKEKEVPAKEKPSFELSGALLEDTNTFRGVVIKYSEPPEARIPKKRWRLYPFKNDEVLPVMYIHRQSAYLLGRHRRIADIPIDHPSCSKQHAVFQYRLVEYTRADGTVGRRVKPYIIDLGSGNGTFLNNKRIEPQRYYELKEKDVLKFGFSSREYVLLHESSDTSEIDRKDDEDEEEEEEVSDS*

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 Smad nuclear interacting protein 1
别名 dJ423B22.2, FLJ12553, RP3 423B22.3, SNIP1
计算分子量 46 kDa
观测分子量 46-50 kDa
GenBank蛋白编号BC027040
基因名称 SNIP1
Gene ID (NCBI) 79753
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ8TAD8
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Smad nuclear-interacting protein 1 (SNIP1) is a novel interacting factor of the Smad protein family that is expressed in different cell types and is involved in cell proliferation, apoptosis, and differentiation.

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