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SNAPC5 Polyclonal antibody

SNAPC5 Polyclonal Antibody for IF/ICC, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, IHC, WB,ELISA

Conjugate

Unconjugated

Cat No : 17272-1-AP

Print datasheet

Synonyms

SNAP19, SNAPc 19 kDa subunit, SNAPc subunit 5, SNAPC5

验证数据展示

  • WB analysis of HeLa using 17272-1-AP

    WB analysis of HeLa using 17272-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 17272-1-AP (SNAPC5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human testis using 17272-1-AP

    IHC staining of human testis using 17272-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 17272-1-AP (SNAPC5 Antibody) at dilution of 1:50.

  • IHC staining of human testis using 17272-1-AP

    IHC staining of human testis using 17272-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 17272-1-AP (SNAPC5 Antibody) at dilution of 1:50.

  • IF Staining of HeLa using 17272-1-AP

    IF Staining of HeLa using 17272-1-AP

    Immunofluorescent analysis of HeLa cells using 17272-1-AP (SNAPC5 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHeLa cells
Positive IHC detected inhuman testis tissue, human breast cancer tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 17272-1-AP is SNAPC5 Fusion Protein expressed in E. coli.

Tested Applications IF/ICC, IHC, WB,ELISA
Cited ApplicationsWB, IF, IHC
Tested Reactivity human
Cited Reactivityhuman
Immunogen SNAPC5 fusion protein Ag11029 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name small nuclear RNA activating complex, polypeptide 5, 19kDa
Synonyms SNAP19, SNAPc 19 kDa subunit, SNAPc subunit 5, SNAPC5
Calculated Molecular Weight68aa,8 kDa; 98aa,11 kDa
Observed Molecular Weight 19 kDa
GenBank Accession NumberBC014315
Gene Symbol SNAPC5
Gene ID (NCBI) 10302
RRIDAB_2878373
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO75971
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SNAPC5, also named as SNAP19, is snRNA-activating protein complex 19 kDa. It's a part of the SNAPc complex required for the transcription of both RNA polymerase II and III small-nuclear RNA genes.

实验方案

Product Specific Protocols
WB protocol for SNAPC5 antibody 17272-1-APDownload protocol
IHC protocol for SNAPC5 antibody 17272-1-APDownload protocol
IF protocol for SNAPC5 antibody 17272-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IHC,IF

Aging (Albany NY)

Exploring Cancer Dependency Map genes and immune subtypes in colon cancer, in which TIGD1 contributes to colon cancer progression

Authors - Guoyang Zhang
View Article