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SMYD1-Specific Polyclonal antibody

SMYD1-Specific Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IP, WB, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 16151-1-AP

Print datasheet

Synonyms

BOP, SMYD1, SMYD1-Specific, ZMYND18, ZMYND22

验证数据展示

  • WB analysis of mouse heart using 16151-1-AP

    WB analysis of mouse heart using 16151-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 16151-1-AP (SMYD1-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human testis using 16151-1-AP

    IHC staining of human testis using 16151-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 16151-1-AP (SMYD1-Specific antibody) at dilution of 1:50 (under 10x lens).

    Ready-to-use IHC kits available with SMYD1 antibody included
  • IHC staining of human testis using 16151-1-AP

    IHC staining of human testis using 16151-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 16151-1-AP (SMYD1-Specific antibody) at dilution of 1:50 (under 40x lens).

    Ready-to-use IHC kits available with SMYD1 antibody included
  • IHC staining of mouse heart using 16151-1-AP

    IHC staining of mouse heart using 16151-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 16151-1-AP (SMYD1-Specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SMYD1 antibody included
  • IHC staining of mouse heart using 16151-1-AP

    IHC staining of mouse heart using 16151-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 16151-1-AP (SMYD1-Specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SMYD1 antibody included
  • IP experiment of mouse heart using 16151-1-AP

    IP experiment of mouse heart using 16151-1-AP

    IP result of anti-SMYD1-Specific (IP:16151-1-AP, 4ug; Detection:16151-1-AP 1:500) with mouse heart tissue lysate 4000ug.

经过测试的应用

Positive WB detected inmouse heart tissue, mouse skeletal muscle tissue
Positive IP detected inmouse heart tissue
Positive IHC detected inhuman testis tissue, human heart tissue, human kidney tissue, human placenta tissue, human skin tissue, human liver tissue, human spleen tissue, human lung tissue, human ovary tissue, mouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy SMYD1 Ready-To-Use IHC Kit. SMYD1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16151-1-AP targets SMYD1-Specific in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IHC, IP, WB, ELISA
Cited ApplicationsWB, IF
Tested Reactivity human, mouse
Cited Reactivitymouse
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name SET and MYND domain containing 1
Synonyms BOP, SMYD1, SMYD1-Specific, ZMYND18, ZMYND22
Calculated Molecular Weight 57 kDa
Observed Molecular Weight 57 kDa
GenBank Accession NumberNM_198274
Gene Symbol SMYD1
Gene ID (NCBI) 150572
RRIDAB_2239747
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8NB12
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SMYD1-Specific antibody 16151-1-APDownload protocol
IHC protocol for SMYD1-Specific antibody 16151-1-APDownload protocol
IP protocol for SMYD1-Specific antibody 16151-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB,IF

Toxicol Lett

Down-regulated Smyd1 participated in the inhibition of myoblast differentiation induced by cigarette smoke extract

Authors - Fang Li
  • KD Validated
View Article