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验证数据展示

  • IHC staining of human colon cancer using 55175-1-AP (same clone as 55175-1-PBS)

    IHC staining of human colon cancer using 55175-1-AP (same clone as 55175-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 55175-1-AP (SMURF1 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 55175-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 55175-1-AP (same clone as 55175-1-PBS)

    IHC staining of human colon cancer using 55175-1-AP (same clone as 55175-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 55175-1-AP (SMURF1 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 55175-1-PBS in a different storage buffer formulation.

  • WB analysis using 55175-1-AP (same clone as 55175-1-PBS)

    WB analysis using 55175-1-AP (same clone as 55175-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 55175-1-AP (SMURF1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55175-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse kidney using 55175-1-AP (same clone as 55175-1-PBS)

    IF Staining of mouse kidney using 55175-1-AP (same clone as 55175-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse kidney tissue using SMURF1 antibody (55175-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55175-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse spleen using 55175-1-AP (same clone as 55175-1-PBS)

    IF Staining of mouse spleen using 55175-1-AP (same clone as 55175-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using SMURF1 antibody (55175-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55175-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

SMURF1 Polyclonal antibody, PBS Only

SMURF1 Polyclonal Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 55175-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF-P, Indirect ELISA

别名

E3 ubiquitin-protein ligase SMURF1, EC:2.3.2.26, HECT-type E3 ubiquitin transferase SMURF1, hSMURF1, KIAA1625

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

55175-1-PBS targets SMURF1 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF-P, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 SMAD specific E3 ubiquitin protein ligase 1
别名 E3 ubiquitin-protein ligase SMURF1, EC:2.3.2.26, HECT-type E3 ubiquitin transferase SMURF1, hSMURF1, KIAA1625
计算分子量 86 kDa
观测分子量90-100 kDa
GenBank蛋白编号NM_020429
基因名称 SMURF1
Gene ID (NCBI) 57154
RRIDAB_10859079
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9HCE7
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SMURF1, also named as KIAA1625, is E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. SMURF1 interacts with receptor-regulated SMADs specific for the BMP pathway, SMAD1 and SMAD5, in order to trigger their ubiquitination and degradation and hence their inactivation. The antibody is specific to SMURF1. SMURF1 can be detected as 90-100 kDa when ubiquitinated (PMID:17576816, PMID:20484049).

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