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SMURF1 Polyclonal antibody
SMURF1 Polyclonal Antibody for IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
IHC, WB, ELISA and More (2)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | mouse testis tissue, rat testis tissue |
Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:8000 |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
55175-1-AP targets SMURF1 in IP, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | IHC, WB, ELISA |
Cited Applications | IP, IHC, CoIP |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat |
Immunogen | Peptide 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | SMAD specific E3 ubiquitin protein ligase 1 |
Synonyms | hSMURF1, KIAA1625, SMURF1 |
Calculated Molecular Weight | 86 kDa |
Observed Molecular Weight | 90-100 kDa |
GenBank Accession Number | NM_020429 |
Gene Symbol | SMURF1 |
Gene ID (NCBI) | 57154 |
RRID | AB_10859079 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q9HCE7 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for SMURF1 antibody 55175-1-AP | Download protocol |
IHC protocol for SMURF1 antibody 55175-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
J Cell Biol A PINCH-1-Smurf1 signaling axis mediates mechano-regulation of BMPR2 and stem cell differentiation. | ||
Free Radic Biol Med CKIP-1 affects the polyubiquitination of Nrf2 and Keap1 via mediating Smurf1 to resist HG-induced renal fibrosis in GMCs and diabetic mice kidneys. | ||
Clin Transl Med MiRNA-516a promotes bladder cancer metastasis by inhibiting MMP9 protein degradation via the AKT/FOXO3A/SMURF1 axis. | ||
J Immunol MLN4924, a First-in-Class NEDD8-Activating Enzyme Inhibitor, Attenuates IFN-β Production.
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Ecotoxicol Environ Saf Exosomal miR-125a-5p derived from silica-exposed macrophages induces fibroblast transdifferentiation. |