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验证数据展示

  • WB analysis of COLO 320 using 14782-1-AP

    WB analysis of COLO 320 using 14782-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 14782-1-AP (SMTNL2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IP experiment of COLO 320 using 14782-1-AP

    IP experiment of COLO 320 using 14782-1-AP

    IP result of anti-SMTNL2 (IP:14782-1-AP, 4ug; Detection:14782-1-AP 1:500) with COLO 320 cells lysate 4000ug.

  • IF Staining of MCF-7 using 14782-1-AP

    IF Staining of MCF-7 using 14782-1-AP

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using SMTNL2 antibody (14782-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

SMTNL2 Polyclonal antibody

SMTNL2 Polyclonal Antibody for WB, IF/ICC, IP, ELISA
Cat No. 14782-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, IP, ELISA

别名

Smoothelin-like protein 2, Smoothelin like protein 2, smoothelin like 2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inCOLO 320 cells, MCF-7 cells, mouse small intestine tissue, SGC-7901 cells
Positive IP detected inCOLO 320 cells
Positive IF/ICC detected inMCF-7 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14782-1-AP targets SMTNL2 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, IP, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag6395

Product name: Recombinant human SMTNL2 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-317 aa of BC112177

Sequence: MRKTSNSCIMENGHQPGTGPGDGPPEIAQNFSAPDPPRPRPVSLSLRLPHQPVTAITRVSDRFSGETSAAALSPMSAATLGGLNPSPSEVITPWTPSPSEKNSSFTWSVPSSGYGAVTASKHSNSPPLVTPPQSPVSPQPPAITQVHRQGERRRELVRSQTLPRTSEAQARKALFEKWEQETAAGKGKGEARARLKRSQSFGVASASSIKQILLEWCRSKTLGYQHVDLQNFSSSWSDGMAFCALVHSFFPDAFDYNSLSPTQRQKNFELAFTMAENLANCERLIEVEDMMVMGRKPDPMCVFTYVQSLYNHLRRFE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 smoothelin-like 2
别名 Smoothelin-like protein 2, Smoothelin like protein 2, smoothelin like 2
计算分子量 50 kDa
观测分子量 68-70 kDa
GenBank蛋白编号BC112177
基因名称 SMTNL2
Gene ID (NCBI) 342527
RRIDAB_2270842
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ2TAL5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SMTNL2 antibody 14782-1-APDownload protocol
IF protocol for SMTNL2 antibody 14782-1-APDownload protocol
IP protocol for SMTNL2 antibody 14782-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols