验证数据展示
经过测试的应用
Positive WB detected in | COLO 320 cells, HEK-293 cells |
Positive IHC detected in | human kidney tissue, human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:500-1:2000 |
Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
16552-1-AP targets SMPDL3B in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
经测试应用 | WB, IHC, ELISA Application Description |
文献引用应用 | WB, IF, IHC |
经测试反应性 | human, mouse, rat |
文献引用反应性 | human, mouse, rat, monkey, pig |
免疫原 | SMPDL3B fusion protein Ag9535 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | sphingomyelin phosphodiesterase, acid-like 3B |
别名 | ASM like phosphodiesterase 3b, ASML3B, SMPDL3B |
计算分子量 | 373aa,42 kDa; 455aa,51 kDa |
观测分子量 | 42 kDa, 50-65 kDa |
GenBank蛋白编号 | BC014444 |
基因名称 | SMPDL3B |
Gene ID (NCBI) | 27293 |
RRID | AB_2878276 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q92485 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SMPDL3B is lipid-modulating phosphodiesterase. Active on the surface of macrophages and dendritic cells and strongly influences macrophage lipid composition and membrane fluidity. SMPDL3B acts as a negative regulator of Toll-like receptor signaling.It lacks activity with phosphocholine-containing lipids, but can cleave CDP-choline, and can release phosphate from ATP and ADP in vitro (PMID: 26095358). SMPDL3B can be detected 60-70 kDa by N-glycosylation modification (PMID: 26095358, PMID: 31217420).
实验方案
Product Specific Protocols | |
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WB protocol for SMPDL3B antibody 16552-1-AP | Download protocol |
IHC protocol for SMPDL3B antibody 16552-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
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Front Mol Biosci SMPDL3B Predicts Poor Prognosis and Contributes to Development of Acute Myeloid Leukemia
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JHEP Rep Macrophage-derived thrombospondin 1 promotes obesity-associated non-alcoholic fatty liver disease. | ||
iScience Porcine reproductive and respiratory syndrome virus upregulates SMPDL3B to promote viral replication by modulating lipid metabolism
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