验证数据展示
经过测试的应用
| Positive WB detected in | NR8383 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
16310-1-AP targets SMPDL3A in WB, ELISA applications and shows reactivity with human, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, rat |
| 免疫原 |
CatNo: Ag9340 Product name: Recombinant human SMPDL3A protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 103-453 aa of BC018999 Sequence: SFMIWTGDSPPHVPVPELSTDTVINVITNMTTTIQSLFPNLQVFPALGNHDYWPQDQLPVVTSKVYNAVANLWKPWLDEEAISTLRKGGFYSQKVTTNPNLRIISLNTNLYYGPNIMTLNKTDPANQFEWLESTLNNSQQNKEKVYIIAHVPVGYLPSSQNITAMREYYNEKLIDIFQKYSDVIAGQFYGHTHRDSIMVLSDKKGSPVNSLFVAPAVTPVKSVLEKQTNNPGIRLFQYDPRDYKLLDMLQYYLNLTEANLKGESIWKLEYILTQTYDIEDLQPESLYGLAKQFTILDSKQFIKYYNYFFVSYDSSVTCDKTCKAFQICAIMNLDNISYADCLKQLYIKHNY 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | sphingomyelin phosphodiesterase, acid-like 3A |
| 别名 | 2',3'-cGAMP phosphodiesterase SMPDL3A, Acid sphingomyelinase-like phosphodiesterase 3a, ASM like phosphodiesterase 3a, ASM3A, ASML3A |
| 计算分子量 | 453 aa, 51 kDa |
| 观测分子量 | 51 kDa |
| GenBank蛋白编号 | BC018999 |
| 基因名称 | SMPDL3A |
| Gene ID (NCBI) | 10924 |
| RRID | AB_3741882 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q92484 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Sphingomyelin phosphodiesterase acid-like 3A (SMPDL3A) is a secreted metallophosphoesterase belonging to the calcineurin-like phosphoesterase superfamily, closely related to acid sphingomyelinase (ASMase). Despite its name, SMPDL3A lacks activity against sphingomyelin and instead functions as a nucleotide phosphodiesterase, hydrolyzing nucleoside di- and triphosphates such as ATP. The enzyme contains a binuclear zinc center in its active site and exhibits an acidic pH optimum. The expression of SMPDL3A is induced by cholesterol loading and liver X receptor (LXR) ligands. Recent studies have identified SMPDL3A as a negative regulator of innate immunity by degrading 2'3'-cGAMP, thereby inhibiting the cGAS-STING signaling pathway.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SMPDL3A antibody 16310-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
