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验证数据展示

  • WB analysis using 86668-2-RR (same clone as 86668-2-PBS)

    WB analysis using 86668-2-RR (same clone as 86668-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86668-2-RR (SMN antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86668-2-PBS in a different storage buffer formulation.

  • IP experiment of HEK-293 using 86668-2-RR (same clone as 86668-2-PBS)

    IP experiment of HEK-293 using 86668-2-RR (same clone as 86668-2-PBS)

    IP result of anti-SMN (IP: 86668-2-RR, 4 ug; Detection: 86668-2-RR 1:600) with HEK-293 cells lysate 1200 ug. This data was developed using the same antibody clone with 86668-2-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 86668-2-RR (same clone as 86668-2-PBS)

    IF Staining of HepG2 using 86668-2-RR (same clone as 86668-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SMN antibody (86668-2-RR, Clone: 251620C4 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86668-2-PBS in a different storage buffer formulation.

  • Sandwich ELISA sample test of MP02580-1

    Sandwich ELISA sample test of MP02580-1

    The mean SMN concentration was determined to be 13.66 ng/mL in HeLa cell extract based on a 1.2 mg/mL extract load.

  • Sandwich ELISA standard curve of MP02580-1

    Sandwich ELISA standard curve of MP02580-1

    Sandwich ELISA standard curve of MP02580-1, Human SMN Recombinant Matched Antibody Pair - PBS only. 86668-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg4812. 86668-1-PBS was HRP conjugated as the detection antibody. Range: 39.1-2500 pg/mL

  • IF Staining of HeLa using 86668-2-RR (same clone as 86668-2-PBS)

    IF Staining of HeLa using 86668-2-RR (same clone as 86668-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SMN antibody (86668-2-RR, Clone: 251620C4 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 86668-2-PBS in a different storage buffer formulation.

  • Cytometric bead array sample test of MP02580-1

    Cytometric bead array sample test of MP02580-1

    The mean SMN concentration was determined to be 4.5 ng/mL in HeLa cell extract based on a 1.1 mg/mL extract load.

  • Cytometric bead array standard curve of MP02580-1

    Cytometric bead array standard curve of MP02580-1

    Cytometric bead array standard curve of MP02580-1, SMN Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 86668-2-PBS. Detection antibody: 86668-1-PBS. Standard: Eg4812. Range: 0.312-40 ng/mL.

SMN Recombinant monoclonal antibody, PBS Only (Capture)

SMN Uni-rAb® Recombinant Antibody for WB, IF/ICC, IP, Cytometric bead array, Sandwich ELISA, Indirect ELISA
Cat No. 86668-2-PBS

产品说明书

CloneNo. 251620C4

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, IP, Cytometric bead array, Sandwich ELISA, Indirect ELISA

别名

C BCD541, Component of gems 1, Gemin 1, Gemin-1, SMN1

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

86668-2-PBS targets SMN as part of a matched antibody pair:

MP02580-1: 86668-2-PBS capture and 86668-1-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IF/ICC, IP, Cytometric bead array, Sandwich ELISA, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Eg4812

Product name: Recombinant Human SMN protein (rFc Tag)

Source: mammalian cells-derived, pHZ-KIsec-C-rFc

Tag: C-rFc

Domain: 90-151 aa of BC015308

Sequence: QQWKVGDKCSAIWSEDGCIYPATIASIDFKRETCVVVYTGYGNREEQNLSDLLSPICEVANN

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 survival of motor neuron 2, centromeric
别名 C BCD541, Component of gems 1, Gemin 1, Gemin-1, SMN1
计算分子量30 kDa
观测分子量38 kDa
GenBank蛋白编号BC015308
基因名称 SMN
Gene ID (NCBI) 6607
RRIDAB_3745042
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ16637
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Spinal muscular atrophy (SMA) is an autosomal recessive neurodegenerative disease characterized by loss of anterior horn cells in the spinal cord and concomitant symmetrical muscle weakness and atrophy (PMID: 16364894 ). SMA is caused by deletion or mutations of the survival motor neuron (SMN1) gene. SMA patients lack a functional SMN1 gene, but they possess an intact SMN2 gene, which though nearly identical to SMN1, is only partially functional (PMID: 17355180). A large majority of SMN2 transcripts lack exon 7, resulting in production of a truncated, less stable SMN protein (PMID: 10369862). The level of SMN protein correlates with phenotypic severity of SMA.

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