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验证数据展示

  • WB analysis using 26153-1-AP (same clone as 26153-1-PBS)

    WB analysis using 26153-1-AP (same clone as 26153-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 26153-1-AP (SMEK1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 26153-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 26153-1-AP (same clone as 26153-1-PBS)

    IHC staining of human colon cancer using 26153-1-AP (same clone as 26153-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 26153-1-AP (SMEK1 antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26153-1-PBS in a different storage buffer formulation.

  • IF Staining of MCF-7 using 26153-1-AP (same clone as 26153-1-PBS)

    IF Staining of MCF-7 using 26153-1-AP (same clone as 26153-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using SMEK1 antibody (26153-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 26153-1-PBS in a different storage buffer formulation.

SMEK1 Polyclonal antibody, PBS Only

SMEK1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 26153-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

KIAA2010, PP4R3A, PPP4R3A

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

26153-1-PBS targets SMEK1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag5070

Product name: Recombinant human SMEK1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 246-594 aa of BC038932

Sequence: LTAPLLANTTEDKPSKDDFQTAQLLALVLELLTFCVEHHTYHIKNYIINKDILRRVLVLMASKHAFLALCALRFKRKIIGLKDEFYNRYIMKSFLFEPVVKAFLNNGSRYNLMNSAIIEMFEFIRVEDIKSLTAHVIENYWKALEDVDYVQTFKGLKLRFEQQRERQDNPKLDSMRSILRNHRYRRDARTLEDEEEMWFNTDEDDMEDGEAVVSPSDKTKNDDDIMDPISKFMERKKLKESEEKEVLLKTNLSGRQSPSFKLSLSSGTKTNLTSQSSTTNLPGSPGSPGSPGSPGSPGSVPKNTSQTAAITTKGGLVGLVDYPDDDEDDDEDEDKEDTLPLSKKAKFDS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 SMEK homolog 1, suppressor of mek1 (Dictyostelium)
别名 KIAA2010, PP4R3A, PPP4R3A
计算分子量 833 aa, 95 kDa
观测分子量95-100 kDa
GenBank蛋白编号BC038932
基因名称 SMEK1
Gene ID (NCBI) 55671
RRIDAB_3742171
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ6IN85
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PPP4R3A also known as SMEK1, is the regulatory subunit of serine/threonine-protein phosphatase 4. SMEK1 regulates the activity of PPP4C at centrosomal microtubule organizing centers. The PPP4C-PPP4R2-PPP4R3A PP4 complex specifically dephosphorylates H2AX phosphorylated on 'Ser-140' (gamma-H2AX) generated during DNA replication and required for DNA DSB repair(PMID: 18614045).

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