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验证数据展示

  • WB analysis using 26153-1-AP

    WB analysis using 26153-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26153-1-AP (SMEK1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 26153-1-AP

    IHC staining of human colon cancer using 26153-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 26153-1-AP (SMEK1 antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of MCF-7 using 26153-1-AP

    IF Staining of MCF-7 using 26153-1-AP

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using SMEK1 antibody (26153-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

SMEK1 Polyclonal antibody

SMEK1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 26153-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA

别名

KIAA2010, PP4R3A, PPP4R3A

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:300-1:1200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26153-1-AP targets SMEK1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 SMEK1 fusion protein Ag5070 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 SMEK homolog 1, suppressor of mek1 (Dictyostelium)
别名 KIAA2010, PP4R3A, PPP4R3A
计算分子量 833 aa, 95 kDa
观测分子量95-100 kDa
GenBank蛋白编号BC038932
基因名称 SMEK1
Gene ID (NCBI) 55671
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ6IN85
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PPP4R3A also known as SMEK1, is the regulatory subunit of serine/threonine-protein phosphatase 4. SMEK1 regulates the activity of PPP4C at centrosomal microtubule organizing centers. The PPP4C-PPP4R2-PPP4R3A PP4 complex specifically dephosphorylates H2AX phosphorylated on 'Ser-140' (gamma-H2AX) generated during DNA replication and required for DNA DSB repair(PMID: 18614045).

实验方案

Product Specific Protocols
WB protocol for SMEK1 antibody 26153-1-APDownload protocol
IHC protocol for SMEK1 antibody 26153-1-APDownload protocol
IF protocol for SMEK1 antibody 26153-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols