验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, HEK-293 cells, A431 cells, MCF-7 cells, NIH/3T3 cells, C6 cells |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
86182-1-RR targets SMC4 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag19156 Product name: Recombinant human SMC4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 992-1288 aa of BC106033 Sequence: SVLIHNSDEHKDIQSCTVEVHFQKIIDKEGDDYEVIPNSNFYVSRTACRDNTSVYHISGKKKTFKDVGNLLRSHGIDLDHNRFLILQGEVEQIAMMKPKGQTEHDEGMLEYLEDIIGCGRLNEPIKVLCRRVEILNEHRGEKLNRVKMVEKEKDALEGEKNIAIEFLTLENEIFRKKNHVCQYYIYELQKRIAEMETQKEKIHEDTKEINEKSNILSNEMKAKNKDVKDTEKKLNKITKFIEENKEKFTQLDLEDVQVREKLKHATSKAKKLEKQLQKDKEKVEEFKSIPAKSNNII 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | structural maintenance of chromosomes 4 |
| 别名 | CAP C, CAPC, Chromosome-associated polypeptide C, hCAP C, hCAPC |
| 计算分子量 | 1288 aa, 147 kDa |
| 观测分子量 | 170-180 kDa |
| GenBank蛋白编号 | BC106033 |
| 基因名称 | SMC4 |
| Gene ID (NCBI) | 10051 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q9NTJ3 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SMC4, also named as Structural maintenance of chromosomes protein 4, is a 1288 amino acid protein, which is widely expressed and belongs to the SMC family, SMC4 subfamily. SMC4 is a Central component of the condensin complex, a complex required for conversion of interphase chromatin into mitotic-like condense chromosomes.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for SMC4 antibody 86182-1-RR | Download protocol |
| WB protocol for SMC4 antibody 86182-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
