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验证数据展示

  • WB analysis using 86182-1-RR (same clone as 86182-1-PBS)

    WB analysis using 86182-1-RR (same clone as 86182-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86182-1-RR (SMC4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86182-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 86182-1-RR (same clone as 86182-1-PBS)

    IF Staining of HeLa using 86182-1-RR (same clone as 86182-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SMC4 antibody (86182-1-RR, Clone: 250782E1 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86182-1-PBS in a different storage buffer formulation.

SMC4 Recombinant monoclonal antibody, PBS Only

SMC4 Uni-rAb® Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 86182-1-PBS

产品说明书

CloneNo. 250782E1

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, Indirect ELISA

别名

CAP C, CAPC, Chromosome-associated polypeptide C, hCAP C, hCAPC

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy SMC4 Ready-To-Use IHC Kit. SMC4 primary antibody included.

产品信息

86182-1-PBS targets SMC4 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag19156

Product name: Recombinant human SMC4 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 992-1288 aa of BC106033

Sequence: SVLIHNSDEHKDIQSCTVEVHFQKIIDKEGDDYEVIPNSNFYVSRTACRDNTSVYHISGKKKTFKDVGNLLRSHGIDLDHNRFLILQGEVEQIAMMKPKGQTEHDEGMLEYLEDIIGCGRLNEPIKVLCRRVEILNEHRGEKLNRVKMVEKEKDALEGEKNIAIEFLTLENEIFRKKNHVCQYYIYELQKRIAEMETQKEKIHEDTKEINEKSNILSNEMKAKNKDVKDTEKKLNKITKFIEENKEKFTQLDLEDVQVREKLKHATSKAKKLEKQLQKDKEKVEEFKSIPAKSNNII

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 structural maintenance of chromosomes 4
别名 CAP C, CAPC, Chromosome-associated polypeptide C, hCAP C, hCAPC
计算分子量 1288 aa, 147 kDa
观测分子量170-180 kDa
GenBank蛋白编号BC106033
基因名称 SMC4
Gene ID (NCBI) 10051
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ9NTJ3
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SMC4, also named as Structural maintenance of chromosomes protein 4, is a 1288 amino acid protein, which is widely expressed and belongs to the SMC family, SMC4 subfamily. SMC4 is a Central component of the condensin complex, a complex required for conversion of interphase chromatin into mitotic-like condense chromosomes.

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