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验证数据展示

  • WB analysis using 84835-2-RR

    WB analysis using 84835-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84835-2-RR (SMC2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84835-2-RR

    Affinity and Kinetic Characterization of 84835-2-RR

    Biolayer interferometry (BLl) kinetic assays of 84835-2-RR against Human SMC2 were performed. The affinity constant is below 1 pM.

SMC2 Recombinant monoclonal antibody

SMC2 Recombinant Antibody for WB, ELISA
Cat No. 84835-2-RR

产品说明书

CloneNo. 241536E1

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, ELISA

别名

SMC protein 2, SMC 2, hCAP E, CAPE, CAP E

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, K-562 cells, Raji cells, HepG2 cells, A431 cells, HEK-293T cells, C2C12 cells
Planning an IHC experiment? We recommend our IHCeasy SMC2 Ready-To-Use IHC Kit. SMC2 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84835-2-RR targets SMC2 in WB, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag33471

Product name: Recombinant human SMC2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1000-1197 aa of NM_001042550.1

Sequence: DLMKKKRIVENDKSKILTTIEDLDQKKNQALNIAWQKVNKDFGSIFSTLLPGANAMLAPPEGQTVLDGLEFKVALGNTWKENLTELSGGQRSLVALSLILSMLLFKPAPIYILDEVDAALDLSHTQNIGQMLRTHFTHSQFIVVSLKEGMFNNANVLFKTKFVDGVSTVARFTQCQNGKISKEAKSKAKPPKGAHVEV

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 structural maintenance of chromosomes 2
别名 SMC protein 2, SMC 2, hCAP E, CAPE, CAP E
计算分子量136 kDa
观测分子量 135-140 kDa
GenBank蛋白编号NM_001042550.1
基因名称 SMC2
Gene ID (NCBI) 10592
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDO95347
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SMC2 is a central component of the condensin complex, a complex required for conversion of interphase chromatin into mitotic-like condense chromosomes. The condensin complex probably introduces positive supercoils into relaxed DNA in the presence of type I topoisomerases and converts nicked DNA into positive knotted forms in the presence of type II topoisomerases (PMID: 11136719).

实验方案

Product Specific Protocols
WB protocol for SMC2 antibody 84835-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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