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SMARCD2 Polyclonal antibody

SMARCD2 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, WB,ELISA

Conjugate

Unconjugated

Cat No : 11156-1-AP

Print datasheet

Synonyms

BAF60B, CRACD2, PRO2451, Rsc6p, SMARCD2

验证数据展示

  • WB analysis of RAW264.7 using 11156-1-AP

    WB analysis of RAW264.7 using 11156-1-AP

    RAW264.7 cells were subjected to SDS PAGE followed by western blot with 11156-1-AP (SMARCD2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human cervical cancer using 11156-1-AP

    IHC staining of human cervical cancer using 11156-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer using 11156-1-AP (SMARCD2 antibody) at dilution of 1:50 (under 10x lens).

经过测试的应用

Positive WB detected inRAW 264.7 cells, RAW264.7 cells
Positive IHC detected inhuman cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11156-1-AP targets SMARCD2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, WB,ELISA
Tested Reactivity human, mouse, rat
Immunogen SMARCD2 fusion protein Ag1630 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily d, member 2
Synonyms BAF60B, CRACD2, PRO2451, Rsc6p, SMARCD2
Calculated Molecular Weight 52 kDa
Observed Molecular Weight 52 kDa
GenBank Accession NumberBC018953
Gene Symbol SMARCD2
Gene ID (NCBI) 6603
RRIDAB_2192145
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ92925
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The SWI/SNF chromatin remodelling complexes are important regulators of transcription; they consist of large multisubunit assemblies containing either Brm or Brg1 as the catalytic ATPase subunit and a variable subset of approximately 10 Brg/Brm-associated factors (BAF) [PMID:20148946]. SMARCD2 is one of BAF60 proteins, which are found in most complexes, is thought to bridge interactions between transcription factors and SWI/SNF complexes. [PMID:9427560]

实验方案

Product Specific Protocols
WB protocol for SMARCD2 antibody 11156-1-APDownload protocol
IHC protocol for SMARCD2 antibody 11156-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols