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验证数据展示

  • WB analysis of RAW264.7 using 11156-1-AP

    WB analysis of RAW264.7 using 11156-1-AP

    RAW264.7 cells were subjected to SDS PAGE followed by western blot with 11156-1-AP (SMARCD2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human cervical cancer using 11156-1-AP

    IHC staining of human cervical cancer using 11156-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer using 11156-1-AP (SMARCD2 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of mouse spleen using 11156-1-AP

    IHC staining of mouse spleen using 11156-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 11156-1-AP (SMARCD2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse spleen using 11156-1-AP

    IHC staining of mouse spleen using 11156-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 11156-1-AP (SMARCD2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat spleen using 11156-1-AP

    IHC staining of rat spleen using 11156-1-AP

    Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 11156-1-AP (SMARCD2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat spleen using 11156-1-AP

    IHC staining of rat spleen using 11156-1-AP

    Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 11156-1-AP (SMARCD2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

SMARCD2 Polyclonal antibody

SMARCD2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 11156-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily D member 2, SMARCD 2, CRACD2, BRG1-associated factor 60B, 60 kDa BRG-1/Brm-associated factor subunit B

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inRAW 264.7 cells, RAW264.7 cells
Positive IHC detected inmouse spleen tissue, human cervical cancer tissue, rat spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11156-1-AP targets SMARCD2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag1630

Product name: Recombinant human SMARCD2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-78 aa of BC018953

Sequence: RDFMLSFSTDPQDFIQEWLRSQRRDLKIITDVIGNPEEERRAAFYHQPWAQEAVGRHIFAKVQQRRQELEQVLGIRLT

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily d, member 2
别名 SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily D member 2, SMARCD 2, CRACD2, BRG1-associated factor 60B, 60 kDa BRG-1/Brm-associated factor subunit B
计算分子量 52 kDa
观测分子量 52 kDa
GenBank蛋白编号BC018953
基因名称 SMARCD2
Gene ID (NCBI) 6603
RRIDAB_2192145
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ92925
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The SWI/SNF chromatin remodelling complexes are important regulators of transcription; they consist of large multisubunit assemblies containing either Brm or Brg1 as the catalytic ATPase subunit and a variable subset of approximately 10 Brg/Brm-associated factors (BAF) [PMID:20148946]. SMARCD2 is one of BAF60 proteins, which are found in most complexes, is thought to bridge interactions between transcription factors and SWI/SNF complexes. [PMID:9427560]

实验方案

Product Specific Protocols
WB protocol for SMARCD2 antibody 11156-1-APDownload protocol
IHC protocol for SMARCD2 antibody 11156-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols