• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 67979-1-Ig

    WB analysis using 67979-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67979-1-Ig (SMARCB1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • IHC staining of mouse stomach using 67979-1-Ig

    IHC staining of mouse stomach using 67979-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 67979-1-Ig (SMARCB1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse stomach using 67979-1-Ig

    IHC staining of mouse stomach using 67979-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 67979-1-Ig (SMARCB1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

SMARCB1 Monoclonal antibody

SMARCB1 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 67979-1-Ig

产品说明书

CloneNo. 1D10A4

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

INI1, hSNFS, hSNF5, BRG1-associated factor 47, BAF47

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inNCCIT cells, LNcaP cells, HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells
Positive IHC detected inmouse stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67979-1-Ig targets SMARCB1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 SMARCB1 fusion protein Ag28911 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily b, member 1
别名 INI1, hSNFS, hSNF5, BRG1-associated factor 47, BAF47
计算分子量 44 kDa
观测分子量40-45 kDa
GenBank蛋白编号NM_003073
基因名称 SMARCB1
Gene ID (NCBI) 6598
RRIDAB_2918728
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ12824
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SMARCB1 is part of a complex that relieves repressive chromatin structures, allowing the transcriptional machinery to access its targets more effectively. The encoded nuclear protein may also bind to and enhance the DNA joining activity of HIV-1 integrase. This gene has been found to be a tumor suppressor and mutations in it have been associated with malignant rhabdoid tumors. Two transcript variants encoding different isoforms have been found for this gene.

实验方案

Product Specific Protocols
WB protocol for SMARCB1 antibody 67979-1-IgDownload protocol
IHC protocol for SMARCB1 antibody 67979-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols