验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, HEK-293 cells |
| Positive IHC detected in | human stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
13066-1-AP targets SMARCA5 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IF, IP |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag3759 Product name: Recombinant human SMARCA5 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 703-1052 aa of BC023144 Sequence: FTMDTESSVYNFEGEDYREKQKIAFTEWIEPPKRERKANYAVDAYFREALRVSEPKAPKAPRPPKQPNVQDFQFFPPRLFELLEKEILFYRKTIGYKVPRNPELPNAAQAQKEEQLKIDEAESLNDEELEEKEKLLTQGFTNWNKRDFNQFIKANEKWGRDDIENIAREVEGKTPEEVIEYSAVFWERCNELQDIEKIMAQIERGEARIQRRISIKKALDTKIGRYKAPFHQLRISYGTNKGKNYTEEEDRFLICMLHKLGFDKENVYDELRQCIRNSPQFRFDWFLKSRTAMELQRRCNTLITLIERENMELEEKEKAEKKKRGPKPSTQKRKMDGAPDGRGRKKKLKL 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 5 |
| 别名 | EC:3.6.4.-, hISWI, hSNF2H, ISWI, SNF2H |
| 计算分子量 | 1052 aa, 122 kDa |
| 观测分子量 | 122 kDa |
| GenBank蛋白编号 | BC023144 |
| 基因名称 | SMARCA5 |
| Gene ID (NCBI) | 8467 |
| RRID | AB_2191854 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O60264 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SMARCA5, also named as SNF2H or WCRF135, is a 1052 amino acid protein, which localizes in nucleus and is overexpressed in CD34-positive erythrocyte progenitor cells in acute myeloid leukemia. SMARCA5 is one of the most conserved chromatin remodeling factors. It exists in a variety of oligosubunit complexes that move DNA with respect to the histone octamer to generate regularly spaced nucleosomal arrays. Smarca5 interacts with different accessory proteins and represents a molecular motor for DNA replication, repair, and transcription.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for SMARCA5 antibody 13066-1-AP | Download protocol |
| IHC protocol for SMARCA5 antibody 13066-1-AP | Download protocol |
| WB protocol for SMARCA5 antibody 13066-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Exp Med Nuclear DEK preserves hematopoietic stem cells potential via NCoR1/HDAC3-Akt1/2-mTOR axis. | ||
Nucleic Acids Res SIRT6 coordinates with CHD4 to promote chromatin relaxation and DNA repair.
| ||
Cell Death Dis USP3 promotes DNA damage response and chemotherapy resistance through stabilizing and deubiquitinating SMARCA5 in prostate cancer | ||
Arterioscler Thromb Vasc Biol Novel Long Noncoding RNA, Macrophage Inflammation-Suppressing Transcript (MIST), Regulates Macrophage Activation During Obesity. |
