验证数据展示
经过测试的应用
| Positive WB detected in | LNCaP cells, PC-3 cells, K-562 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
88105-1-RR targets SMAP2 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag9751 Product name: Recombinant human SMAP2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 77-429 aa of BC021133 Sequence: QIQCMQEMGNGKANRLYEAYLPETFRRPQIDPAVEGFIRDKYEKKKYMDRSLDINAFRKEKDDKWKRGSEPVPEKKLEPVVFEKVKMPQKKEDPQLPRKSSPKSTAPVMDLLGLDAPVACSIANSKTSNTLEKDLDLLASVPSPSSSGSRKVVGSMPTAGSAGSVPENLNLFPEPGSKSEEIGKKQLSKDSILSLYGSQTPQMPTQAMFMAPAQMAYPTAYPSFPGVTPPNSIMGSMMPPPVGMVAQPGASGMVAPMAMPAGYMGGMQASMMGVPNGMMTTQQAGYMAGMAAMPQTVYGVQPAQQLQWNLTQMTQQMAGMNFYGANGMMNYGQSMSGGNGQAANQTLSPQMWK 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | small ArfGAP2 |
| 别名 | small ArfGAP2, SMAP1L, Stromal membrane-associated protein 1-like, Stromal membrane-associated protein 2 |
| 计算分子量 | 429 aa, 47 kDa |
| 观测分子量 | 47 kDa |
| GenBank蛋白编号 | BC021133 |
| 基因名称 | SMAP2 |
| Gene ID (NCBI) | 64744 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q8WU79 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SMAP2 (Small Arf GAP Protein 2) is an Arf1 GTPase-activating protein. It localizes to early endosomes/TGN, binds clathrin/AP-1, and regulates retrograde transport from recycling endosomes to the Golgi. Critical for vesicle trafficking, organelle homeostasis, and spermatogenesis. (PMID: 26136365)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SMAP2 antibody 88105-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
