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验证数据展示

  • WB analysis using 85388-1-RR

    WB analysis using 85388-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85388-1-RR (SMAD1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • FC experiment of HeLa using 85388-1-RR

    FC experiment of HeLa using 85388-1-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug SMAD1 Recombinant antibody (85388-1-RR, Clone:242844B4) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 85388-1-RR

    Affinity and Kinetic Characterization of 85388-1-RR

    Biolayer interferometry (BLl) kinetic assays of 85388-1-RR against Human SMAD1 were performed. The affinity constant is below 1 pM.

SMAD1 Recombinant antibody

SMAD1 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 85388-1-RR

产品说明书

CloneNo. 242844B4

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, FC (Intra), ELISA

别名

JV4-1, JV41, hSMAD1, BSP-1, BSP1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, HEK-293 cells, HUVEC cells, HT-1080 cells, RAW264.7 cells, HSC-T6 cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy SMAD1 Ready-To-Use IHC Kit. SMAD1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

85388-1-RR targets SMAD1 in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, FC (Intra), ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 SMAD1 fusion protein Ag0701 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 SMAD family member 1
别名 JV4-1, JV41, hSMAD1, BSP-1, BSP1
计算分子量 52 kDa
观测分子量55 kDa
GenBank蛋白编号BC001878
基因名称 SMAD1
Gene ID (NCBI) 4086
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ15797
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Transforming growth factor-β (TGF-β) superfamily is recognized as one of the largest families of secreted multifunctional peptides exerting different biological effects on a large variety of cell types, such as regulation of hormone secretion, stimulation of extracellular matrix formation, the inhibition of proliferation of many cell types, cell survival, bone formation, and chemotaxis for inflammatory cells. One of the most important proteins that modulate TGF-β ligand activity is the SMAD family proteins. SMAD1 is one of the receptor-activated Smads. It's also a signal transducers of BMP signaling and binds to several proteins involved in ubiquitin-proteasome system (UPS). Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase.

实验方案

Product Specific Protocols
WB protocol for SMAD1 antibody 85388-1-RRDownload protocol
FC protocol for SMAD1 antibody 85388-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols