验证数据展示
经过测试的应用
| Positive WB detected in | DU 145 cells, HeLa cells, mouse testis tissue, rat testis tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
31867-1-AP targets SLX4 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag36095 Product name: Recombinant human BTBD12 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1530-1834 aa of BC036335 Sequence: MPSAGGAQKPEGLETPKGANRKKNLPPKVPITPMPQYSIMETPVLKKELDRFGVRPLPKRQMVLKLKEIFQYTHQTLDSDSEDESQSSQPLLQAPHCQTLASQTYKPSRAGVHAQQEATTGPGAHRPKGPAKTKGPRHQRKHHESITPPSRSPTKEAPPGLNDDAQIPASQESVATSVDGSDSSLSSQSSSSCEFGAAFESAGEEEGEGEVSASQAAVQAADTDEALRCYIRSKPALYQKVLLYQPFELRELQAELRQNGLRVSSRRLLDFLDTHCITFTTAATRREKLQGRRRQPRGKKKVERN 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | BTB (POZ) domain containing 12 |
| 别名 | BTBD12, BTB (POZ) domain containing 12, KIAA1784, KIAA1987 |
| 计算分子量 | 1834 aa, 200 kDa |
| 观测分子量 | 180-200 kDa |
| GenBank蛋白编号 | BC036335 |
| 基因名称 | BTBD12 |
| Gene ID (NCBI) | 84464 |
| RRID | AB_3742487 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity Purification |
| UNIPROT ID | Q8IY92 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SLX4 (Structure-specific endonuclease subunit SLX4) is a scaffold protein that assembles multiple structure-specific nucleases, including SLX1, MUS81-EME1, and XPF-ERCC1. These nucleases are essential for resolving complex DNA structures that arise during DNA replication, repair, and recombination (PMID: 34804132). SLX4 is a key protein involved in DNA repair and genome stability, with significant implications for genetic disorders and cancer.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SLX4 antibody 31867-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
