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验证数据展示

  • WB analysis of mouse thymus using 13315-1-AP

    WB analysis of mouse thymus using 13315-1-AP

    mouse thymus tissue were subjected to SDS PAGE followed by western blot with 13315-1-AP (SLD5 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 13315-1-AP

    IF Staining of HeLa using 13315-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SLD5 antibody (13315-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

SLD5 Polyclonal antibody

SLD5 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 13315-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, ELISA

别名

GINS4, GINS complex subunit 4, DNA replication complex GINS protein SLD5, N-terminally processed

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse thymus tissue, mouse testis tissue
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13315-1-AP targets SLD5 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原 SLD5 fusion protein Ag4158 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 GINS complex subunit 4 (Sld5 homolog)
别名 GINS4, GINS complex subunit 4, DNA replication complex GINS protein SLD5, N-terminally processed
计算分子量 223 aa, 26 kDa
观测分子量 26 kDa
GenBank蛋白编号BC027454
基因名称 SLD5
Gene ID (NCBI) 84296
RRIDAB_2109671
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9BRT9
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SLD5 (DNA replication complex GINS protein SLD5) is also named as GINS4 (GINS complex subunit 4). SLD5 is required for correct functioning of the GINS complex, a complex that plays an essential role in the initiation of DNA replication, and progression of DNA replication forks (PMID: 17417653). SLD5 forms a GINS complex with PSF1, PSF2 and PSF3, which is essential for the initiation of DNA replication in lower eukaryotes (PMID: 24244394). SLD5 is a member of the GINS complex, essential for DNA replication in eukaryotes. It has been reported that SLD5 is involved in early embryogenesis in the mouse, and cell cycle progression and genome integrity in Drosophila (PMID: 27499248).

实验方案

Product Specific Protocols
WB protocol for SLD5 antibody 13315-1-APDownload protocol
IF protocol for SLD5 antibody 13315-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols